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Profiling of heat shock proteins 27 and 70 in adenoids of children

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Purpose

Heat shock protein (HSP)27 and 70 are molecular chaperones that may have immunomodulatory functions. We determined if and at what levels each are expressed in the adenoids of pediatric subjects. We also examined tissue distributions, associated clinical characteristics, and antibacterial effects.

Methods

Western blot, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were applied to adenoidal tissues and lavage fluids obtained from children (N = 40) undergoing adenotonsillectomy.

Results

Via western blot and ELISA, both HSP27 and 70 were regularly detected in adenoidal tissue and in lavage fluid samples. HSP27 was highly expressed in epithelium, whereas HSP70 showed strong subepithelial positivity and bore a significant relation to adenoidal size. Assayed levels of HSP27 and 70 correlated inversely, and their addition to culture media independently increased bacterial numbers (Staphylococcus aureus). Upon the precipitation of each from adenoidal lavage fluids, bacterial counts declined.

Conclusions

HSP27 and 70 are readily expressed in the adenoids of children and may be implicated in immunologic responses.

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Acknowledgements

This research received support from the following sources: a National Research Foundation (NRF) grant, funded by the Korean government (MSIP) (NRF- 2017R1A1A1A05000760 to Hyun Jin Min). This study was also supported (in part) by research grant from Biomedical Research Institute, Chung-Ang University Hospital (2018).

Funding

This research received support from the following sources: a National Research Foundation (NRF) grant, funded by the Korean government (MSIP) (NRF- 2017R1A1A1A05000760 to Hyun Jin Min). This study was also supported (in part) by research grant from Biomedical Research Institute, Chung-Ang University Hospital (2018).

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Authors and Affiliations

  1. Department of Otorhinolaryngo-Logy-Head and Neck Surgery, Chung-Ang University Hospital, College of Medicine, 224-1 Heukseok-dong, Dongjak-gu, Seoul, 156-755, Republic of Korea

    Hyun Jin Min, Joon Soon Park, Chang Eurn Kim & Kyung Soo Kim

  2. Chung-Ang University Hospital, Biomedical Research Institute, 224-1 Heukseok-dong, Dongjak-gu, Seoul, 156-755, Republic of Korea

    Hyun Jin Min

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  1. Hyun Jin Min
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  2. Joon Soon Park
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  3. Chang Eurn Kim
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  4. Kyung Soo Kim
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Contributions

HJM wrote the manuscript, JSP performed experiments. CEK was responsible for data collection, and KSK reviewed whole manuscript.

Corresponding author

Correspondence to Kyung Soo Kim.

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Conflict of interest

The authors have no conflicts of interest to declare.

Ethical approval

This study was approved by the Institutional Review Board of Chung-Ang University College of Medicine (C2015092[1550]).

Informed consent

Informed consent was obtained from all of our enrolled subjects.

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Min, H.J., Park, J.S., Kim, C.E. et al. Profiling of heat shock proteins 27 and 70 in adenoids of children. Eur Arch Otorhinolaryngol 276, 2483–2489 (2019). https://doi.org/10.1007/s00405-019-05528-z

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  • DOI: https://doi.org/10.1007/s00405-019-05528-z

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