An investigation of oxidative stress and coenzyme Q10 levels in patients with head and neck squamous cell carcinomas

Abstract

Objective

The purpose of this study was to determine the oxidative states of head and neck squamous cell carcinoma (HNSCC) patients by measuring their plasma levels of malondialdehyde (MDA), an indicator of lipid peroxidation, 3-nitrotyrosine (3-NT), an indicator of protein oxidation, and the coenzyme Q10 (CoQ10), an important antioxidant, and compare them with healthy individuals.

Materials and methods

The plasma MDA, 3-NT and CoQ10 levels of 35 patients and 20 healthy individuals were measured with the high-performance liquid chromatography (HPLC) method. By comparing the patients’ smoking habits, stage of the disease, size of the primary tumor and the presence of lymph nodes and the values of healthy individuals, the oxidative stress load of HNSCC patients was determined.

Results

The mean plasma MDA levels of carcinoma patients were two times higher than those of healthy individuals (p < 0.001). When the mean plasma 3-NT levels of patients and healthy individuals were compared, no significant difference was found (p > 0.05). The mean plasma CoQ10 level of patients was low when compared with healthy individuals; however, no significant difference was detected (p > 0.05). In addition, as the stage and tumor size increased in HNSCC patients, their non-enzymatic antioxidant levels significantly decreased (p < 0.05).

Conclusions

In HNSCC patients, lipo-oxidative damage increased while nitrosative stress did not change; however, antioxidant activity decreased which in turn increased both lipid peroxidation and oxidative stress. These findings support the contention that oxidative stress strongly reflects the health status of HNSCC patients.

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Acknowledgements

This study was submitted as an oral presentation at the 35th Turkish National Otolaryngology—Head and Neck Surgery Congress on Novemder 2–6, 2013, in Antalya, Turkey. The authors thank Gregory Thomas Sullivan of the University of Queensland in Brisbane, Australia, for editing the English in an earlier version of this manuscript.

Funding

This study was funded as a Ondokuz Mayıs University Scientific Research Projects (projectnumber - TIP1904.12.030).

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Correspondence to Ahmet Aksoy.

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Ethical approval

Ethics committee approval (2012/07) was received from Ondokuz Mayis University, Faculty of Medicine. All procedures performed in this study that involved human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Aksoy, A., Kurnaz, S. An investigation of oxidative stress and coenzyme Q10 levels in patients with head and neck squamous cell carcinomas. Eur Arch Otorhinolaryngol 276, 1197–1204 (2019). https://doi.org/10.1007/s00405-019-05328-5

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Keywords

  • Squamous cell carcinoma
  • Coenzyme Q10
  • Malondialdehyde
  • 3-Nitrotyrosine
  • Oxidative stress