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Effects of oral ısotretinoin on normal and wounded nasal mucosa: an experimental study

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Background

We aimed to investigate the effect of systemic isotretinoin therapy on normal and wounded nasal septal mucosa histopathologically in an experimental rabbit model.

Methods

Circular mucosal defect with a 7 mm diameter was made in the left septum of 12 New Zealand white rabbits. The rabbits were divided into two groups (six rabbits in each group) oral isotretinoin was given with olive oil at the operation day to the first group. The control group was only oil given group. The harvested septum mucosas were divided into four groups (1—wounded-drug given side, 2—unwounded and drug-given side, 3—wounded-control and 4—unwounded-control side). The diameter of the defect, mucosal thickness, epithelial thickness, ciliated cell level, goblet cell level and inflammation were evaluated every week for 4 weeks.

Results

At both wounded and normal side, thinning of normal respiratory ciliated epithelium was observed in the postoperative period. In study group at the wounded side; mean mucosal thickness was measured 139.66 µ (± 26.24), and in the control group, mean mucosal thickness was 238.33 µ (± 39.7) at the wounded side. (p < 0.001). The difference between the groups in thickness of normal septal mucosa was also statistically significant (p = 0.016) [190 µ (± 14.6) and 256.66 µ (± 44.66)]. The average cilia level was observed 1.16 in the wounded study group, while the average level was 2.33 in the wounded control group (p = 0.012). Average score measurements of the regenerated mucosa suggested that isotretinoin–given wounded animals have reduced goblet cell recovery, compared to the control both on the regenerated and unwounded mucosas (p = 0.007, p = 0.002, respectively). Inflammation was significantly higher in the wounded isotretinoin group (p = 0.018).

Conclusion

Oral isotretinoin has negative effects on epithelial and ciliary regeneration, significantly reduces mucosal thickness and goblet cell counts of the normal and regenerated mucosa, causes severe inflammation and significant reactive changes.

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The authors declare that they have no funding.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Bagcilar Training and Research Hospital, Saglik Bakanliği University, Merkez Mahallesi, 6. Sk, Bağcilar, 34100, Istanbul, Turkey

    Abdurrahman Bugra Cengiz, Cemal Ozyilmaz, Alper Tabaru, Sertug Sinan Ege, Sedat Ruzgar & Mehmet Faruk Oktay

  2. Department of Otorhinolaryngology, Aksaray University , Aksaray, Turkey

    Serkan Kayabasi

  3. Department of Pathology, Saglik Bakanliği University, Bagcilar Training and Research Hospital, Istanbul, Turkey

    Funda Emre

Authors
  1. Abdurrahman Bugra Cengiz
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  2. Cemal Ozyilmaz
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Correspondence to Abdurrahman Bugra Cengiz.

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All procedures performed in studies involving animals were in accordance with the ethical standards of Institutional Animal Care and Use Committee in Bagcilar Training and Research Hospital. This article does not contain any studies with human participants performed by any of the authors.

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Cengiz, A.B., Ozyilmaz, C., Tabaru, A. et al. Effects of oral ısotretinoin on normal and wounded nasal mucosa: an experimental study. Eur Arch Otorhinolaryngol 275, 3025–3031 (2018). https://doi.org/10.1007/s00405-018-5190-2

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  • DOI: https://doi.org/10.1007/s00405-018-5190-2

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