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Effects of polymer-based, silver nanoparticle-coated silicone splints on the nasal mucosa of rats

European Archives of Oto-Rhino-Laryngology volume 274, pages 1535–1541 (2017)Cite this article

Abstract

Infection is a serious complication after nasal packing that otolaryngologists seek to avoid. The aim of this study is to investigate the use of silver (Ag) nanoparticle, which serves as antimicrobial agents, with nasal tampons. The study design is an experimental animal model and the setting is tertiary referral center. Twenty-four rats were randomized into the following four groups: (1) control group (n = 6); (2) silicone nasal splint (SNS) group (n  =  6); (3) polypropylene-grafted polyethylene glycol (PP-g-PEG) amphiphilic graft copolymer-coated SNS group (n  =  6); and (4) Ag nanoparticle-embedded PP-g-PEG (Ag-PP-g-PEG) amphiphilic graft copolymer-coated SNS group (n  =  6). These tampons were applied to rats for 48 h, after which they were removed in a sterile manner, and the rats were sacrificed. The nasal septa of the rats were excised, and assessments of tissue changes in the nasal mucosa were compared among the groups. The removed tampons were microbiologically examined, and quantitative analyses were made. When the groups were compared microbiologically, there were no significant differences in bacterial colonization rates of coagulase-negative Staphylococcus spp. among the three groups (p = 0.519), but there was a statistically significant difference among bacterial colonization rates of Heamophilus parainfluenzae and Corynebacterium spp. (p = 0.018, p = 0.004). We found that H. parainfluenzae grew less robustly in the Ag-PP-g-PEG than the PP-g-PEG group (p = 0.017). However, we found no significant difference between the Ag-PP-g-PEG and SNS groups, or between the SNS and PP-g-PEG groups. The growth of Corynebacterium spp. did not differ significantly between the Ag-PP-g-PEG and SNS groups (p = 1.000). When Group 4 was compared with Group 2, the former showed less inflammation. Compared with other tampons, Ag-PP-g-PEG amphiphilic graft copolymer-coated silicone nasal tampons caused less microbiological colonization and inflammation. Therefore, the use of these tampons may prevent secondary infections and reduce the risk of developing complications by minimizing tissue damage.

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References

  1. Korkut AY, Teker AM, Eren SB, Gedikli O, Askiner O (2010) A randomised prospective trial of trans-septal suturing using a novel device versus nasal packing for septoplasty. Rhinology 48:179–182

    PubMed  Google Scholar 

  2. Ardehali MM, Bastaninejad S (2009) Use of nasal packs and intranasal septal splints following septoplasty. Int J Oral Maxillofac Surg 38:1022–1024

    CAS  Article  PubMed  Google Scholar 

  3. Cayonu M, Acar A, Horasanlı E, Altundag A, Salihoglu M (2014) Comparison of totally occlusive nasal pack, internal nasal splint, and transseptal suture technique after septoplasty in terms of immediate respiratory distress related to anesthesia and surgical complications. Acta Otolaryngol 13:390–394

    Article  Google Scholar 

  4. Tan M, Kalcioglu MT, Sahin N, Bayindir T, Samdanci E, Filiz A (2015) Assessment of mucosal changes associated with nasal splint in a rabbit model. Braz J Otorhinolaryngol 81:184–189

    Article  PubMed  Google Scholar 

  5. Wang Y, Chen S, Chen J, Zhang W, Gong G, Zhou T, Kong W (2013) Bacterial biofilm formation after nasal packing in nasal mucosa-wounded mice. Am J Rhinol Allergy 27:91–95

    Article  PubMed  Google Scholar 

  6. Şereflican M, Yurttaş V, Oral M, Yılmaz B, Dağlı M (2015) Is middle ear pressure effected by nasal packings after septoplasty? J Int Adv Otol 11:63–65

    Article  PubMed  Google Scholar 

  7. Yilmaz MS, Guven M, Buyukarslan DG, Kaymaz R, Erkorkmaz U (2012) Do silicone nasal septal splints with integral airway reduce postoperative eustachian tube dysfunction? Otolaryngol Head Neck Surg 146:141–145

    Article  PubMed  Google Scholar 

  8. Schweitzer DH, Moffie BG, van der Mey AG, Thompson J (1990) A patient with toxic shock syndrome following correction of the nasal septum. Ned Tijdschr Geneeskd 134:2146–2148

    CAS  PubMed  Google Scholar 

  9. Nahass RG, Gocke DJ (1988) Toxic shock syndrome associated with use of a nasal tampon. Am J Med 84:629–631

    CAS  Article  PubMed  Google Scholar 

  10. Ahmed Q, Gupta N, Kumar A, Nimesh S (2016) Antibacterial efficacy of silver nanoparticles synthesized employing Terminalia arjuna bark extract. Artif Cells Nanomed Biotechnol 9:1–9

    Article  Google Scholar 

  11. Balcı M, Allı A, Hazer B, Güven O, Cavicchi K, Çakmak M (2010) Synthesis and characterization of novel comb-type amphiphilic graft copolymers containing polypropylene and polyethylene glycol. Polym Bull 64:691–705

    Article  Google Scholar 

  12. Kalaycı ÖA, Cömert FB, Hazer B, Atalay T, Cavicchi K, Çakmak M (2010) Synthesis, characterization, and antibacterial activity of metal nanoparticles embedded into amphiphilic comb-type graft copolymers. Polym Bull 65:215–226

    Article  Google Scholar 

  13. Garcia LS, Isenberg HD (2010) Wound cultures. In: Clinical microbiology procedures handbook. 3rd edn. ASM Press, Washington, DC, pp 441–462

  14. Jallah Z, Liang R, Feola A, Barone W, Palcsey S, Abramowitch SD, Yoshimura N, Moalli P (2016) The impact of prolapse mesh on vaginal smooth muscle structure and function. BJOG 123:1076–1085

    CAS  Article  PubMed  Google Scholar 

  15. Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E (2015) Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution. Int J Nanomed 10:2635–2646

    CAS  Article  Google Scholar 

  16. Chue WL, Campbell GR, Caplice N, Muhammed A, Berry CL, Thomas AC, Bennett MB, Campbell JH (2004) Dog peritoneal and pleural cavities as bioreactors to grow autologous vascular grafts. J Vasc Surg 39:859–867

    Article  PubMed  Google Scholar 

  17. Gogoi D, Choudhury AJ, Chutia J, Pal AR, Khan M, Choudhury M, Pathak P, Das G, Patil DS (2014) Development of advanced antimicrobial and sterilized plasma polypropylene grafted muga (Antheraea assama) silk as suture biomaterial. Biopolymers 101:355–365

    CAS  Article  PubMed  Google Scholar 

  18. Scheidbach H, Tamme C, Tannapfel A, Lippert H, Köckerling F (2003) In vivo studies comparing the biocompatibility of various polypropylene meshes and their handling properties during endoscopic total extraperitoneal (TEP) patchplasty: an experimental study in pig. Surg Endosc 18:211–220

    Article  PubMed  Google Scholar 

  19. Harris JM (ed) (1992) Poly(ethylene glycol) chemistry: biotechnical and biomedical applications. Plenum Press, New York

    Google Scholar 

  20. Li X, Loh XJ, Li J (2005) Poly (ester urethane) s consisting of poly [(R)-3-hydroxybutyrate] and poly(ethylene glycol) as candidate biomaterials: characterization and mechanical property study. Biomacromolecules 6:2740–2747

    CAS  Article  PubMed  Google Scholar 

  21. Markowska K, Grudniak AM, Wolska KI (2013) Silver nanoparticles as an alternative strategy against bacterial biofilms. Acta Biochim Pol 60:523–530

    PubMed  Google Scholar 

  22. Rai M, Kon K, Ingle A et al (2014) Broad-spectrum bioactivities of silver nanoparticles: the emerging trends and future prospects. Appl Microbiol Biotechnol 98:1951–1961

    CAS  Article  PubMed  Google Scholar 

  23. Hazer DB, Mut M, Dincer N et al (2012) The efficacy of silver-embedded polypropylene-grafted polyethylene glycol-coated ventricular catheters on prevention of shunt catheter infection in rats. Childs Nerv Syst 28:839–846

    Article  PubMed  Google Scholar 

  24. Nair LS, Laurencin CT (2008) Nanofibers and nanoparticles for orthopedic surgery applications. J Bone Jt Surg Am 90:128–131

    Article  Google Scholar 

  25. Savolainen S, Ylikoski J, Jousimies-Somer H (1986) The bacterial flora of the nasal cavity in healthy young men. Rhinology 24:249–255

    CAS  PubMed  Google Scholar 

  26. Hazer DB, Sakar M, Dere Y, Altinkanat G, Ziyal MI, Hazer B (2016) Antimicrobial effect of polymer-based silver nanoparticle coated pedicle screws: experimental research on biofilm inhibition in rabbits. Spine (Phila Pa 1976) 41:323–329

    Article  Google Scholar 

  27. Middleton AM, Dowling RB, Mitchell JL, Watanabe S, Rutman A, Pritchard K, Tillotson G, Hill SL, Wilson R (2003) Haemophilus parainfluenzae infection of respiratory mucosa. Respir Med 97:375–381

    CAS  Article  PubMed  Google Scholar 

  28. Wald ER (1988) Sinusitis in children. Pediatr Infect Dis J 7:150–153

    Article  Google Scholar 

  29. Biswas K, Hoggard M, Jain R, Taylor MW, Douglas RG (2015) The nasal microbiota in health and disease: variation within and between subjects. Front Microbiol 9:134

    Article  PubMed  Google Scholar 

  30. Liu Q, Lu X, Bo M, Qing H, Wang X, Zhang L (2014) The microbiology of chronic rhinosinusitis with and without nasal polyps. Acta Otolaryngol 134:1251–1258

    CAS  Article  PubMed  Google Scholar 

  31. Kim RJ, Biswas K, Hoggard M, Taylor MW, Douglas RG (2015) Paired analysis of the microbiota of surface mucus and whole-tissue specimens in patients with chronic rhinosinusitis. Int Forum Allergy Rhinol 2(5):877–883

    Article  Google Scholar 

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Acknowledgements

We would to thank Assistant Professor Fürüzan Köktürk, from the Biostatistics department of Bülent Ecevit University Faculty of Medicine, for performing the statistical analyses in this study; and Nilüfer Ugur Özlük, from the Microbiology department of Bülent Ecevit University Faculty of Medicine, for the microbiological analysis.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey

    Sultan Şevik Eliçora, Duygu Erdem & Aykut Erdem Dinç

  2. Department of Physics, Faculty of Arts and Science, Bülent Ecevit University, Zonguldak, Turkey

    Özlem Altunordu Kalaycı

  3. Department of Chemistry, Engineering of Nanotechnology, Bülent Ecevit University, Zonguldak, Turkey

    Baki Hazer

  4. Department of Pathology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey

    Gamze Yurdakan

  5. Department of Microbiology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey

    Canan Külah

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  1. Sultan Şevik Eliçora
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  2. Duygu Erdem
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  4. Özlem Altunordu Kalaycı
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Correspondence to Sultan Şevik Eliçora.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Şevik Eliçora, S., Erdem, D., Dinç, A.E. et al. Effects of polymer-based, silver nanoparticle-coated silicone splints on the nasal mucosa of rats. Eur Arch Otorhinolaryngol 274, 1535–1541 (2017). https://doi.org/10.1007/s00405-016-4394-6

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  • DOI: https://doi.org/10.1007/s00405-016-4394-6

Keywords

  • Polyethylene glycol
  • Ag nanoparticle
  • Nasal tampon
  • Nasal splint