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Effect of transtympanic betamethasone delivery to the inner ear

  • Otology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

To investigate the effect of transtympanic betamethasone administration on hearing function with histologic correlation, rats were divided into three transtympanic treatment groups: isotonic saline (group I, n = 10), gentamicin (group II, n = 10) and betamethasone (group III, n = 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (p > 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (p < 0.05) and group III (p < 0.05). In the Terminal deoxynucleotidyl transferase dUTP Nick End Labelling (TUNEL), Caspase-3, Caspase-8 and Caspase-9 staining method the amount of apoptotic cells in group II were significantly elevated in all measurements compared with group I (p < 0.05). In the TUNEL staining method the amount of apoptotic cells in Group III were significantly elevated compared with group I in both the organ of Corti and spiral ganglion neurons (p < 0.05). The overall histological results revealed that the severity of cellular apoptosis caused by betamethasone was somewhere between isotonic saline and gentamicin. Transtympanic betamethasone does not affect inner ear function as measured by distortion product otoacoustic emission responses, but some increase in cellular apoptosis in the organ of Corti and spiral ganglion neurons was observed. These findings suggest that transtympanic betamethasone may have mild ototoxic effects. Further studies are needed to obtain precise results for transtympanic application of betamethasone.

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Acknowledgments

We thank Si-Ser Hearing Center for their support for distortion product-evoked otoacoustic emission testing.

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Authors and Affiliations

  1. Department of Otolaryngology, Kocaeli Derince Research and Training Hospital, Kocaeli Derince Eğitim ve Araştırma Hastanesi Kulak Burun Boğaz Kliniği, 41900, Derince, Kocaeli, Turkey

    Halil Erdem Özel, Fatih Özdoğan, Erkan Esen, Adin Selçuk & Selahattin Genç

  2. Department of Histology and Embryology, Celal Bayar University School of Vocational Health Service, Manisa, Turkey

    Seren Gülşen Gürgen

Authors
  1. Halil Erdem Özel
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  2. Fatih Özdoğan
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  3. Seren Gülşen Gürgen
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  4. Erkan Esen
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  5. Adin Selçuk
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  6. Selahattin Genç
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Correspondence to Halil Erdem Özel.

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We have no conflict of interest.

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All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

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Kocaeli Derince Research and Training Hospital has provided financial support for this work.

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Özel, H.E., Özdoğan, F., Gülşen Gürgen, S. et al. Effect of transtympanic betamethasone delivery to the inner ear. Eur Arch Otorhinolaryngol 273, 3053–3061 (2016). https://doi.org/10.1007/s00405-016-3905-9

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  • DOI: https://doi.org/10.1007/s00405-016-3905-9

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