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Brush biopsy with DNA-image cytometry: a useful and noninvasive method for monitoring malignant transformation of potentially malignant oral disorders

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Abstract

Oral and pharyngeal cancer is the sixth most common cancer worldwide, the 5-year survival rate has not yet increased. A key factor in rates not having improved is the lack of early detection. This study was undertaken to estimate the diagnostic accuracy of brush biopsy with DNA-image cytometry (a noninvasive method) for potentially malignant oral disorders compared with tissue biopsy pathology in China. Exfoliative cells were obtained using a cytobrush cell collector from oral mucosa of 52 subjects, followed by scalpel biopsy from the same region. Nuclear DNA contents (ploidy) were measured after Feulgen restaining, using an automated DNA image cytometer. Exfoliative cytology with DNA-image cytometry and histopathological diagnosis were performed separately at different institutions. Histological investigation was considered the gold standard. We reported that the sensitivity of DNA aneuploidy for the detection of cancer cells in potentially malignant oral disorders was 86.36 %, its specificity was 90.00 %, its positive predictive value was 86.36 %, and its negative predictive value was 90.00 %. Brush biopsy with DNA-image cytometry is a useful method for monitoring potentially malignant oral disorders.

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (No. 81170961) and project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (No. PAPD 2011–2013). The automated DNA image cytometer was supplied by WUHAN LANDING MEIDICAL HIGN-TECH CO., LTD. The authors would like to express their appreciation to them.

Ethical approval

The study was approved by the Ethical Committee of Stomatological Hospital Affiliated to Nanjing Medical University (No. PJ2011-012-03). The authors declare that our studies have been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and that all persons gave their informed consent prior to their inclusion in the study.

Conflict of interest

The authors declare that they have no conflicts of interests.

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Authors and Affiliations

  1. Institute of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People’s Republic of China

    Jiang-Min Ma, Ting-Jun Zhou, Ran Wang, Jing Shan, Yu-Nong Wu, Xiao-Ling Song, Ning Gu & Yuan Fan

  2. Department of Oral Medicine, School of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People’s Republic of China

    Jiang-Min Ma, Ting-Jun Zhou, Ran Wang, Jing Shan & Yuan Fan

  3. Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People’s Republic of China

    Yu-Nong Wu

  4. Department of Oral Pathology, School of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People’s Republic of China

    Xiao-Ling Song

  5. Department of Clinical Laboratory, School of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People’s Republic of China

    Ning Gu

Authors
  1. Jiang-Min Ma
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  2. Ting-Jun Zhou
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  3. Ran Wang
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  4. Jing Shan
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  5. Yu-Nong Wu
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  6. Xiao-Ling Song
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  7. Ning Gu
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  8. Yuan Fan
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Corresponding author

Correspondence to Yuan Fan.

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Ma, JM., Zhou, TJ., Wang, R. et al. Brush biopsy with DNA-image cytometry: a useful and noninvasive method for monitoring malignant transformation of potentially malignant oral disorders. Eur Arch Otorhinolaryngol 271, 3291–3295 (2014). https://doi.org/10.1007/s00405-014-2935-4

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  • DOI: https://doi.org/10.1007/s00405-014-2935-4

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