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Expression of the cysteinyl leukotriene 1 receptor and glucocorticoid receptor-β in nasal polyps

  • Rhinology
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Abstract

The objective of this study was to analyze the expression of cysteinyl leukotriene 1 (CysLT1) receptor and glucocorticoid receptors (GRs) in nasal polyps, and to evaluate the relationship between the expression of CysLT1 receptors and that of GRs. Nasal polyps were taken from 32 patients of chronic rhinosinusitis with nasal polyposis. Samples of middle turbinate from seven healthy subjects were used as controls. Specimens were immunohistochemically stained for CysLT1 receptor, GR-α and GR-β receptor, and were quantified in the unit area of the tissues. Numbers of CysLT1 receptor-positive cells were much increased in nasal polyps than in middle turbinate (281 ± 67 vs. 157 ± 85 cells/mm2, P = .01). There was no significant difference in the numbers of GR-α positive cells between nasal polyps and normal turbinate mucosa. GR-β positive cells were increased in nasal polyps as compared to normal turbinate mucosa (36 ± 8 vs. 19 ± 7 cells/mm2, P = .03). A significant relationship was found between the expression of CysLT1 receptor and GR-β in nasal polyps (R = .525, P = .04), whereas there was no significant relationship between the expression of CysLT1 receptor and GR-α in nasal polyps. Our study shows that CysLT1 receptor expression predominates on GR-β over-expressed polyps. This may suggest the additional effect of CysLT1 receptor antagonist for the treatment of nasal polyposis resistant to steroid alone.

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References

  1. Mygind N (1990) Nasal polyposis. J Allergy Clin Immunol 86:827–829

    Article  PubMed  CAS  Google Scholar 

  2. Hamilos DL, Leung DY, Muro S et al (2001) GRbeta expression in nasal polyp inflammatory cells and its relationship to the anti-inflammatory effects of intranasal fluticasone. J Allergy Clin Immunol 108:59–68

    Article  PubMed  CAS  Google Scholar 

  3. Wang M, Shi P, Chen B, Shi G, Li H, Wang H (2011) Superantigen-induced glucocorticoid insensitivity in the recurrence of chronic rhinosinusitis with nasal polyps. Otolaryngol Head Neck Surg 145:717–722

    Article  PubMed  Google Scholar 

  4. Christodoulopoulos P, Cameron L, Durham S, Hamid Q (2000) Molecular pathology of allergic disease II: upper airway disease. J Allergy Clin Immunol 105:211–223

    Article  PubMed  CAS  Google Scholar 

  5. Currie GP, Lee DK, Srivastava P (2005) Long-acting bronchodilator or leukotriene modifier as add-on therapy to inhaled corticosteroids in persistent asthma? Chest 128:2954–2962

    Article  PubMed  CAS  Google Scholar 

  6. Ducharme FM (2011) Leukotriene receptor antagonists as first line or add-on treatment for asthma. BMJ 13:343

    Google Scholar 

  7. Nagata M, Saito K (2003) The roles of cysteinyl leukotrienes in eosinophilic inflammation of asthmatic airways. Int Arch Allergy Immunol 131:7–10

    Article  PubMed  CAS  Google Scholar 

  8. Chao SS, Graham SM, Brown CL, Kline JN, Hussain I (2006) Cysteinyl leukotriene 1 receptor expression in nasal polyps. Ann Otol Rhinol Laryngol 115:394–397

    PubMed  Google Scholar 

  9. Rasp G (2010) Is there a role for leukotriene antagonists in the prevention of recurrent nasal polyps? Curr Opin Allergy Clin Immunol 10:200–205

    Article  PubMed  CAS  Google Scholar 

  10. Hui Y, Funk CD (2002) Cysteinyl leukotriene receptors. Biochem Pharmacol 64:1549–1557

    Article  PubMed  CAS  Google Scholar 

  11. Lund VJ, Mackay IS (1993) Staging in rhinosinusitis. Rhinology 31:183–184

    PubMed  CAS  Google Scholar 

  12. Goleva E, Li LB, Eves PT, Strand MJ, Martin RJ, Leung DY (2006) Increased glucocorticoid receptor beta alters steroid response in glucocorticoid-insensitive asthma. Am J Respir Crit Care Med 173:607–616

    Article  PubMed  CAS  Google Scholar 

  13. Sousa AR, Parikh A, Scadding G, Corrigan CJ, Lee TH (2002) Leukotriene-receptor expression on nasal mucosal inflammatory cells in aspirin-sensitive rhinosinusitis. N Engl J Med 347:1524–1526

    Article  Google Scholar 

  14. Shirasaki H, Kanaizumi E, Watanabe K et al (2002) Expression and localization of the cysteinyl leukotriene 1 receptor in human nasal mucosa. Clin Exp Allergy 32:1007–1012

    Article  PubMed  CAS  Google Scholar 

  15. Adamusiak AM, Stasikowska-Kanicka O, Lewandowska-Polak A et al (2011) Expression of arachidonate metabolism enzymes and receptors in nasal polyps of aspirin-hypersensitive asthmatics. Int Arch Allergy Immunol 157:354–362

    Article  PubMed  Google Scholar 

  16. Oakley RH, Jewell CM, Yudt MR, Bofetiado DM, Cidlowski JA (1999) The dominant negative activity of the human glucocorticoid receptor beta isoform. Specificity and mechanisms of action. J Biol Chem 274:27857–27866

    Article  PubMed  CAS  Google Scholar 

  17. Pujols L, Mullol J, Benítez P et al (2003) Expression of the glucocorticoid receptor alpha and beta isoforms in human nasal mucosa and polyp epithelial cells. Respir Med 97:90–96

    Article  PubMed  Google Scholar 

  18. Fakhri S, Tulic M, Christodoulopoulos P et al (2004) Microbial superantigens induce glucocorticoid receptor beta and steroid resistance in a nasal explant model. Laryngoscope 114:887–892

    Article  PubMed  Google Scholar 

  19. de Wang Y (2008) Significance of susceptible gene expression profiles in nasal polyposis. Clin Exp Otorhinolaryngol 1:177–183

    Article  Google Scholar 

  20. Hamid QA, Wenzel SE, Hauk PJ et al (1999) Increased glucocorticoid receptor beta in airway cells of glucocorticoid-insensitive asthma. Am J Respir Crit Care Med 159:1600–1604

    PubMed  CAS  Google Scholar 

  21. Sousa AR, Lane SJ, Cidlowski JA, Staynov DZ, Lee TH (2000) Glucocorticoid resistance in asthma is associated with elevated in vivo expression of the glucocorticoid receptor b-isoform. J Allergy Clin Immunol 105:943–950

    Article  PubMed  CAS  Google Scholar 

  22. Honda M, Orii F, Ayabe T et al (2000) Expression of glucocorticoid receptor beta in lymphocytes of patients with glucocorticoid-resistant ulcerative colitis. Gastroenterology 118:859–866

    Article  PubMed  CAS  Google Scholar 

  23. Shahidi H, Vottero A, Stratakis CA et al (1999) Imbalanced expression of the glucocorticoid receptor isoforms in cultured lymphocytes from a patient with systemic glucocorticoid resistance and chronic lymphocytic leukemia. Biochem Biophys Res Commun 254:559–565

    Article  PubMed  CAS  Google Scholar 

  24. Gagliardo R, Chanez P, Vignola AM et al (2000) Glucocorticoid receptor alpha and beta in glucocorticoid dependent asthma. Am J Respir Crit Care Med 162:7–13

    PubMed  CAS  Google Scholar 

  25. Pujols L, Alobid I, Benítez P et al (2008) Regulation of glucocorticoid receptor in nasal polyps by systemic and intranasal glucocorticoids. Allergy 63:1377–1386

    Article  PubMed  CAS  Google Scholar 

  26. Watanabe S, Suzaki H (2008) Changes of glucocorticoid receptor expression in the nasal polyps of patients with chronic sinusitis following treatment with glucocorticoid. In Vivo 22:37–42

    PubMed  CAS  Google Scholar 

  27. Yu M, Watanabe S, Kudo M, Kanai K, Suzaki H (2009) Glucocorticoid receptor immunoreactivity of eosinophils in nasal polyps. Acta Otolaryngol Suppl 562:95–100

    Article  PubMed  CAS  Google Scholar 

  28. Parnes SM, Chuma AV (2000) Acute effects of antileukotrienes on sinonasal polyposis and sinusitis. Ear Nose Throat J 79(18–20):24–25

    Google Scholar 

  29. Stewart RA, Ram B, Hamilton G, Weiner J, Kane KJ (2008) Montelukast as an adjunct to oral and inhaled steroid therapy in chronic nasal polyposis. Otolaryngol Head Neck Surg 139:682–687

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Departments of Otorhinolaryngology, Seoul National University College of Medicine, Seoul National University Hospital, 101 Daehangno, Jongno-gu, Seoul, 110-744, Korea

    Yong-Hwi An, Sung-Lyong Hong, Doo Hee Han, Chul Hee Lee, Yang-Gi Min & Chae-Seo Rhee

  2. Departments of Otorhinolaryngology, Eulji University School of Medicine, Seoul, Korea

    Yong-Hwi An

  3. Departments of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine, Pusan, Korea

    Sung-Lyong Hong

  4. Research Center for Sensory Organs, Seoul National University Medical Research Center, Seoul, Korea

    Doo Hee Han, Chul Hee Lee, Yang-Gi Min & Chae-Seo Rhee

  5. Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea

    Chae-Seo Rhee

  6. Department of Immunology, Graduate School of Medicine, Seoul, Korea

    Chae-Seo Rhee

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  1. Yong-Hwi An
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  2. Sung-Lyong Hong
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Correspondence to Chae-Seo Rhee.

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An, YH., Hong, SL., Han, D.H. et al. Expression of the cysteinyl leukotriene 1 receptor and glucocorticoid receptor-β in nasal polyps. Eur Arch Otorhinolaryngol 270, 1373–1378 (2013). https://doi.org/10.1007/s00405-012-2239-5

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  • DOI: https://doi.org/10.1007/s00405-012-2239-5

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