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European Archives of Oto-Rhino-Laryngology

, Volume 270, Issue 4, pp 1203–1208 | Cite as

Oxidative stress in chronic otitis media

  • Elif Baysal
  • Nurten Aksoy
  • Ferit Kara
  • Seyithan Taysi
  • Abdullah Taşkın
  • Hasan Bilinç
  • Cengiz Cevik
  • Fatih Celenk
  • Muzaffer Kanlıkama
Otology

Abstract

Chronic otitis media usually presents with a benign tumor-like lesion of the temporal bone known as a cholesteatoma. The role of oxidative stress in the pathogenesis of chronic otitis media and cholesteatoma has not yet been fully explored. Therefore, the aim of this study was to investigate the oxidative stress markers and antioxidant enzymes in patients with cholesteatomatous and noncholesteatomatous chronic otitis media and in healthy subjects. A prospective controlled trial was performed on cholesteatomatous and noncholesteatomatous chronic otitis media patients in a tertiary referral center in a university hospital. A total of 75 subjects, including 25 cholesteatomatous and 25 noncholesteatomatous chronic otitis media patients and 25 healthy subjects participated in this study. Serum total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly increased in the patient groups with or without cholesteatoma compared with the control group. Serum total antioxidant status (TAS) levels and Paraoxonase and arylesterase activity were significantly lower in the patient groups with or without cholesteatoma compared with the control group. Serum TOS and OSI levels were lower in the noncholesteatomatous group, whereas serum TAS levels were higher compared with the cholesteatomatous group. Serum arylesterase activity was significantly lower in the noncholesteatomatous group compared with the control group. The results of this study reveal that in cholesteatoma cases, the oxidative stress and antioxidant enzyme imbalance were more significant than in cases of chronic otitis media without cholesteatoma.

Keywords

Chronic otitis media Cholesteatoma Oxidative stress Paraoxonase Prolidase 

References

  1. 1.
    Louw L (2010) Acquired cholesteatoma pathogenesis: stepwise explanations. J Laryngol Otol 124:587–593PubMedCrossRefGoogle Scholar
  2. 2.
    Olszewska E, Wagner M, Bernal-Sprekelsen M, Ebmeyer J, Dazert S, Hildmann H, Sudhoff H (2004) Etiopathogenesis of cholesteatoma. Eur Arch Otorhinolaryngol 261:6–24PubMedCrossRefGoogle Scholar
  3. 3.
    Huisman MA, deHeer E, Dijke PT, Grote JJ (2007) Transforming growth factor β and wound healing in human cholesteatoma. Laryngoscope 118:94–98CrossRefGoogle Scholar
  4. 4.
    Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, Al B, Ozabacigil F, Yilmaz A, Tahsin Ozder H (2008) Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. Int J Radiat Biol 84:803–808PubMedCrossRefGoogle Scholar
  5. 5.
    Aktan B, Gundogdu C, Ucuncu H, Unal B, Sutbeyaz Y, Altas S (2004) Anti-inflammatory effect of erythromycin on histamine-induced otitis media with effusion in guinea pigs. J Laryngol Otol 118:97–101PubMedCrossRefGoogle Scholar
  6. 6.
    Okumus S, Taysi S, Orkmez M, Saricicek E, Demir E, Adli M, Al B (2011) The effects of oral ginkgo biloba supplementation on radiation-induced oxidative injury in the lens of rat. Pharmacogn Mag 7:141–145PubMedCrossRefGoogle Scholar
  7. 7.
    Koc S, Aksoy N, Bilinc H, Duygu F, Uysal IO, Ekinci A (2011) Paraoxonase and arylesterase activity and total oxidative/anti-oxidative status in patients with chronic adenotonsillitis. Int J Pediatr Otorhinolaryngol 75:1364–1367PubMedCrossRefGoogle Scholar
  8. 8.
    Seres I, Pragh G, Deschene E, Fulop T Jr, Khalil A (2004) Study of factors influencing the decreased HDL associated PON1 activity with aging. Exp Gerontol 39:59–66PubMedCrossRefGoogle Scholar
  9. 9.
    Eckerson HW, Wyte MC, La Du BN (1983) The human serum paroxonase/arylesterase polymorphism. Am J Hum Genet 35:1126–1138PubMedGoogle Scholar
  10. 10.
    Haagen L, Brock A (1992) A new automated method for phenotyping arylesterase (E.C.3.1.1.2.) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clim Biochem 30:391–395Google Scholar
  11. 11.
    Myara I, Charpentier C, Lemonnier A (1982) Optimal conditions for prolidase assay by proline colorimetric determination: application to imminodipeptiduria. Clin Chim Acta 125:193–205PubMedCrossRefGoogle Scholar
  12. 12.
    Chinard FP (1952) Photometric estimation of proline and ornithine. J Biol Chem 199:91–95PubMedGoogle Scholar
  13. 13.
    Erel O (2004) A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 37:112–119PubMedCrossRefGoogle Scholar
  14. 14.
    Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38:1103–1111PubMedCrossRefGoogle Scholar
  15. 15.
    Harma M, Harma M, Erel O (2003) Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly 133:563–566PubMedGoogle Scholar
  16. 16.
    Yumru M, Savas HA, Kalenderoglu A, Bulut M, Celik H, Erel O (2009) Oxidative imbalance in bipolar disorder subtypes: a comparative study. Prog Neuropsychopharmacol Biol Psychiatry 33:1070–1074PubMedCrossRefGoogle Scholar
  17. 17.
    Kosecik M, Erel O, Sevinc E, Selek S (2005) Increased oxidative stress in children exposed to passive smoking. Int J Cardiol 100:61–64PubMedCrossRefGoogle Scholar
  18. 18.
    Aktan B, Taysi S, Gumustekin K, Uçüncü H, Memişoğullari R, Save K, Bakan N (2003) Effect of macrolide antibiotics on nitric oxide synthase and xanthine oxidase activities, and malondialdehyde level in erythrocyte of the guinea pigs with experimental otitis media with effusion. Pol J Pharmacol 55:1105–1110PubMedGoogle Scholar
  19. 19.
    Ece A, Atamer Y, Gürkan F, Davutoğlu M, Bilici M, Tutanç M (2006) Paraoxonase, anti-oxidant response and oxidative stress in children with chronic renal failure. Pediatr Nephrol 21:239–245PubMedCrossRefGoogle Scholar
  20. 20.
    Yılmaz T, Koçan EG, Besler HT (2004) The role of antioxidants in chronic tonsillitis and adenoid hypertrophy in children. Int J Pediatr Otorhinolaryngol 68:1053–1058PubMedCrossRefGoogle Scholar
  21. 21.
    Schulpis KH, Barzelitou M, Papadakis M, Rodolakis A, Antsaklis A, Papassotiriou I, Vlachos GD (2008) Maternal chronic hepatitis B virus is implicated with low neonatal paraoxonase/arylesterase activities. Clin Biochem 41:282–287PubMedCrossRefGoogle Scholar
  22. 22.
    Selek S, Coşar N, Koçyiğit A, Erel O, Aksoy N, Gencer M, Gunak F, Aslan M (2008) PON1 activity and total oxidant status in patients with active pulmonary tuberculosis. Clin Biochem 41:140–144PubMedCrossRefGoogle Scholar
  23. 23.
    Çakmak A, Zeyrek D, Atas A, Selek S, Erel O (2009) Oxidative status and paraoxonase activity in children with asthma. Clin Invest Med 32:327–334Google Scholar
  24. 24.
    Shih DM, Lusis AJ (2009) The roles of PON1 and PON2 in cardiovascular disease and innate immunity. Curr Opin Lipidol 20:288–292PubMedCrossRefGoogle Scholar
  25. 25.
    Tanimoto N, Kumon Y, Suehiro T, Ohkubo S, Ikeda Y, Nishiya K, Hashimoto K (2003) Serum paraoxonase activity decreases in rheumatoid arthritis. Life Sci 72:2877–2885PubMedCrossRefGoogle Scholar
  26. 26.
    Kiss E, Seres I, Tarr T, Kocsis Z, Szegedi G, Paragh G (2007) Reduced paraoxonase1 activity is a risk for atherosclerosis in patients with systemic lupus erythematosus. Ann NY Acad Sci 1108:83–91PubMedCrossRefGoogle Scholar
  27. 27.
    Toker A, Kadi M, Yildirim AK, Aksoy H, Akcay F (2009) Serum lipid profile paraoxonase and arylesterase activities in psoriasis. Cell Biochem Funct 27:176–180PubMedCrossRefGoogle Scholar
  28. 28.
    Karakucuk S, Baskol G, Oner AO, Baskol M, Mirza E, Ustdal M (2004) Serum paraoxonase activity is decreased in the active stage of behcet’s disease. Br J Ophthalmol 88:1256–1258PubMedCrossRefGoogle Scholar
  29. 29.
    Shigemi H, Egashira T, Kurono Y, Mogi G (1998) Role of superoxide dismutase in otitis media with effusion. Ann Otol Rhinol Laryngol 107:327–331PubMedGoogle Scholar
  30. 30.
    Yariktas M, Doner F, Dogru H, Yasan H, Delibas N (2004) The role of free oxygen radicals on the development of otitis media with effusion. Int J Pediatr Otorhinolaryngol 68:889–894PubMedCrossRefGoogle Scholar
  31. 31.
    Hamzei M, Ventriglia G, Hagnia M, Antonopolous A, Bernal-Sprekelsen M, Dazert S, Hildmann H, Sudhoff H (2003) Osteoclast stimulating and differentiating factors in human cholesteatoma. Laryngoscope 113:436–442PubMedCrossRefGoogle Scholar
  32. 32.
    Chole RA, Faddis BT (2002) Evidence for microbial biofilms in cholesteatomas. Arch Otolaryngol Head Neck Surg 128:1129–1133PubMedGoogle Scholar
  33. 33.
    Jung JY, Pashia ME, Nishimoto SY, Faddis BT, Chole RA (2004) A possible role for nitric oxide in osteoclastogenesis associated with cholesteatoma. Otol Neurotol 25:661–668PubMedCrossRefGoogle Scholar
  34. 34.
    Camps J, Pujol I, Ballester F, Joven J, Simó JM (2011) Paraoxonases as potential antibiofilm agents: their relationship with quorum-sensing signals in gram-negative bacteria. Antimicrob Agents Chemother 55:1325–1331PubMedCrossRefGoogle Scholar
  35. 35.
    Cakmak A, Zeyrek D, Atas A, Celik H, Aksoy N, Erel O (2009) Serum prolidase activity and oxidative status in patients with bronchial asthma. J Clin Lab Anal 23:132–138PubMedCrossRefGoogle Scholar
  36. 36.
    Powell GF, Kurosky A, Maniscalco RM (1977) Prolidase deficiency: report of a second case with quantitation of the excessively excreted amino acids. J Pediatr 91:242–246Google Scholar
  37. 37.
    Amar MS, Wishahi HF, Zakhary MM (1996) Clinical and biochemical studies of bone destruction in cholesteatoma. J Laryngol Otol 110:534–539PubMedCrossRefGoogle Scholar
  38. 38.
    Aberg B, Bagger-Sjöbäck D, Edström S, Heyden G, Jacobsson M, Kindblom LG (1990) A comparative study of enzyme histochemical features in the gerbilline and human cholesteatoma. Acta Otolaryngol 109:130–146PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Elif Baysal
    • 1
  • Nurten Aksoy
    • 2
  • Ferit Kara
    • 3
  • Seyithan Taysi
    • 4
  • Abdullah Taşkın
    • 2
  • Hasan Bilinç
    • 2
  • Cengiz Cevik
    • 5
  • Fatih Celenk
    • 1
  • Muzaffer Kanlıkama
    • 1
  1. 1.Department of Otolaryngology, Medical FacultyGaziantep UniversityGaziantepTurkey
  2. 2.Department of Biochemistry, Medical FacultyHarran UniversitySanliurfaTurkey
  3. 3.Department of OtolaryngologyKahta State HospitalAdiyamanTurkey
  4. 4.Department of Biochemistry, Medical FacultyGaziantep UniversityGaziantepTurkey
  5. 5.Department of Otolaryngology, Medical FacultyMustafa Kemal UniversityHatayTurkey

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