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Anti-cholinergic effect of singulair on isolated rat’s tracheal smooth muscle

  • Rhinology
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Abstract

Singulair (Montelukast) is a potent and selective leukotriene D4 receptor antagonist, often used in treating inflammatory conditions of the respiratory system such as allergic rhinitis and asthma. However, the effects of singulair given intratracheally have rarely been well explored. To verify the effect of singulair, which acts on the tracheal smooth muscle directly in vitro. We used our preparation to test the effects of singulair on isolated rat’s tracheal smooth muscle. The following assessments of singulair were performed: (1) effect on the tracheal smooth muscle resting tension, (2) effect on contraction caused by 10−6 M methacholine as a parasympathetic mimetic, and (3) effect of the drugs on electrically induced tracheal smooth muscle contractions. The results indicated that the addition of methacholine to the incubation medium caused the trachea to contract in a dose-dependent manner. Addition of singulair at doses of 10−5 M or above elicited a significant relaxation response to 10−6 M methacholine-induced contraction. Singulair could not inhibit electrical field stimulation-induced spike contraction. It also had a minimal effect on the basal tension of trachea as the concentration increased. This study showed that the high concentrations of singulair also had an anti-cholinergic effect for relieving symptoms of asthma.

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Acknowledgments

This work was supported in part by Taipei Medical University, Shuang-Ho Hospital (100MTU-SHH-05).

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Authors and Affiliations

  1. Graduate Institute of Clinical Medicine and Department of Otolaryngology, College of Medicine, Taipei Medical University, Shuang Ho Hospital, No. 291, Jhong-Jheng Road, Jhonghe, Taipei County, Taiwan

    Hsing-Won Wang

  2. Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Gong Road, Neihu District, Taipei, Taiwan

    Li-Hsiang Cheng, Chuan-Hsiang Kao, Chih-Hung Wang, Yueng-Hsiang Chu & Hsing-Won Wang

  3. Graduate Institute of Medical Sciences and Department of Physiology, College of Medicine, Taipei Medical University, Taipei, Taiwan

    Jia-Yi Wang

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  1. Li-Hsiang Cheng
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  2. Chuan-Hsiang Kao
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  3. Chih-Hung Wang
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  4. Yueng-Hsiang Chu
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  5. Jia-Yi Wang
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  6. Hsing-Won Wang
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Correspondence to Hsing-Won Wang.

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Cheng, LH., Kao, CH., Wang, CH. et al. Anti-cholinergic effect of singulair on isolated rat’s tracheal smooth muscle. Eur Arch Otorhinolaryngol 269, 1923–1927 (2012). https://doi.org/10.1007/s00405-011-1880-8

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  • DOI: https://doi.org/10.1007/s00405-011-1880-8

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