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Impact of ozone exposure on prostaglandin release in nasal polyps

  • Rhinology
  • Published:
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Abstract

A dysregulation of the cyclooxygenases and a leukotriene/prostaglandin imbalance are assumed to be part of the pathogenesis of the aspirin (ASA) intolerance syndrome. Ozone is an air pollutant with known proinflammatory effects on exposed epithelia, however, its impact on the expression of the cyclooxygenases 1 and 2 (cox1/2) and prostaglandin E2 (PGE2) in the nasal mucosa is not well known. Therefore, we analyzed cox expression and PGE2 levels after ozone exposure in nasal mucosa and in nasal polyps considering ASA intolerance. Isolated epithelial nasal cells from control subjects without chronic rhinosinusitis (CRS), and those from patients with nasal polyps with and without ASA intolerance were cultured and exposed in vitro to ozone. Cox1/2 expression levels were analyzed by immunohistochemistry and PGE2 release by ELISA. After ozone exposure cox1/2 expression remained unchanged in all the three groups. PGE2 release was lowered in cell cultures from controls and from polyps of ASA tolerant but not in those of ASA intolerant patients after ozone exposure. In the latter, PGE2 expression remained unchanged. Our in vitro data suggest that aspirin tolerant patients with polyps might be more affected by ozone compared to aspirin intolerant ones. The potential clinical impact of impaired PGE2 expression caused by ozone on the functions of respiratory epithelia remains to be clarified.

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Acknowledgments

We are greatly indebted to Ms. Karin Bakes and Ms. Bettina Mros for their excellent technical support.

Conflict of interest

No conflicts of interest to declare.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstr. 1, 55101, Mainz, Germany

    Cheng Jing Zhu, Kai Fruth, Wolf J. Mann & Juergen Brieger

  2. Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI) at the University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany

    Astrid Schneider

Authors
  1. Cheng Jing Zhu
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  2. Kai Fruth
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  3. Astrid Schneider
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  4. Wolf J. Mann
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  5. Juergen Brieger
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Corresponding author

Correspondence to Kai Fruth.

Additional information

C. J. Zhu and K. Fruth have contributed equally to this work.

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Zhu, C.J., Fruth, K., Schneider, A. et al. Impact of ozone exposure on prostaglandin release in nasal polyps. Eur Arch Otorhinolaryngol 269, 1623–1628 (2012). https://doi.org/10.1007/s00405-011-1856-8

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  • DOI: https://doi.org/10.1007/s00405-011-1856-8

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