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Effects of hyperbaric oxygen treatment on auditory hair cells after acute noise damage

  • Otology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Acute acoustic trauma (AAT) is a sudden sensorineural hearing loss caused by exposure of the hearing organ to acoustic overstimulation, typically an intense sound impulse, hyperbaric oxygen therapy (HOT), which favors repair of the microcirculation, can be potentially used to treat it. Hence, this study aimed to assess the effects of HOT on guinea pigs exposed to acoustic trauma. Fifteen guinea pigs were exposed to noise in the 4-kHz range with intensity of 110 dB sound level pressure for 72 h. They were assessed by brainstem auditory evoked potential (BAEP) and by distortion product otoacoustic emission (DPOAE) before and after exposure and after HOT at 2.0 absolute atmospheres for 1 h. The cochleae were then analyzed using scanning electron microscopy (SEM). There was a statistically significant difference in the signal-to-noise ratio of the DPOAE amplitudes for the 1- to 4-kHz frequencies and the SEM findings revealed damaged outer hair cells (OHC) after exposure to noise, with recovery after HOT (p = 0.0159), which did not occur on thresholds and amplitudes to BAEP (p = 0.1593). The electrophysiological BAEP data did not demonstrate effectiveness of HOT against AAT damage. However, there was improvement of the anatomical pattern of damage detected by SEM, with a significant reduction of the number of injured cochlear OHC and their functionality detected by DPOAE.

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Abbreviations

AAT:

Acute acoustic trauma

ATA:

Absolute atmospheric pressure

OHC:

Outer hair cells

HOT:

Hyperbaric oxygen therapy

FMRP:

Faculty of Medicine of Ribeirão Preto

USP:

University of São Paulo

OAE:

Otoacoustic emission

DPOAE:

Distortion product otoacoustic emission

BEAP:

Brainstem auditory evoked potential

dB SPL:

Decibel sound pressure level

SEM:

Scanning electron microscopy

T1:

Basal turn

T2:

Second turn

T3:

Third turn

SOD:

Superoxide dismutase

SOD1:

Copper/zinc-superoxide dismutase

R1:

First row

R2:

Second row

R3:

Third row

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Acknowledgments

The research related to this article has been sponsored by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) under the process of number 2007/59213-2.

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Authors and Affiliations

  1. Division of Otorhinolaryngology, Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Prêto, SP, 14049-900, Brazil

    Gleice Cristina Colombari, Maria Rossato, Omar Feres & Miguel Angelo Hyppolito

  2. Division of Surgery, Department of Anatomy and Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Prêto, Brazil

    Omar Feres

Authors
  1. Gleice Cristina Colombari
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  2. Maria Rossato
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  3. Omar Feres
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  4. Miguel Angelo Hyppolito
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Correspondence to Miguel Angelo Hyppolito.

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Colombari, G.C., Rossato, M., Feres, O. et al. Effects of hyperbaric oxygen treatment on auditory hair cells after acute noise damage. Eur Arch Otorhinolaryngol 268, 49–56 (2011). https://doi.org/10.1007/s00405-010-1338-4

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  • DOI: https://doi.org/10.1007/s00405-010-1338-4

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