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Metal-proteinase ADAM12, kinesin 14 and checkpoint suppressor 1 as new molecular markers of laryngeal carcinoma

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Abstract

The assessment of gene expression profile in laryngeal cancer allows implementation of molecular biology methods in diagnostics, as well as in prognosticating the course of disease, thus allowing taking most optimal decisions as regards the method of treatment, scope of surgical procedure, or the necessity of adding complementary radiotherapy. The aim of the project was to analyze the gene expression profile in laryngeal cancer using oligonucleotide microarrays, having in mind searching new molecular markers for that carcinoma. The study comprised a group of 43 patients (38 males and 5 females) suffering from squamous cell laryngeal carcinoma, diagnosed and surgically treated in the years 2005–2007 in the ENT Department of the Silesian Medical University in Katowice, Poland. RNA was isolated from frozen tissue fragments, with the use of columns RNeasy Midi and Mini Kit (Qiagen). For the examination of gene expression profile, oligonucleotide microarrays of high density were used, provided by Affymetrix (U 133 2.0 PLUS) containing over 54,000 probes for over 47,000 transcripts. Four genes previously not examined in that respect in laryngeal carcinoma, occurred to be good markers of the neoplasm. They are: metal-proteinase ADAM12, cyclin-dependent kinase 2–CDK2, kinesin 14–KIF14, suppressor 1 of checkpoint–CHES1. The analysis of gene expression profile allows, in laryngeal carcinoma, to point out to new genes, which in future may become molecular markers of the carcinoma.

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Acknowledgments

Project financed by grant of Polish Ministry of Science and Higher Education No. 3 PO5B 11225. We acknowledge the help of Aleksandra Pfeifer MSc, in the preparation of figures.

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Authors and Affiliations

  1. ENT Department, Silesian Medical University, Francuska Str. 20-24, 40-270, Katowice, Poland

    Jarosław Markowski, Tatiana Gierek, Małgorzata Witkowska & Jarosław Paluch

  2. Department of Nuclear Medicine and Endocrine Oncology, Maria Skłodowska–Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

    Tomasz Tyszkiewicz, Małgorzata Oczko-Wojciechowska, Małgorzata Kowalska, Zbigniew Wygoda & Barbara Jarząb

  3. Department of Clinical and Experimental Oncology, Dept. of Tumor Biology, Maria Skłodowska–Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

    Michał Jarząb

  4. Department of Pathology, Maria Skłodowska–Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

    Dariusz Lange

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  1. Jarosław Markowski
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  2. Tomasz Tyszkiewicz
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  3. Michał Jarząb
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  4. Małgorzata Oczko-Wojciechowska
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  6. Małgorzata Witkowska
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  7. Jarosław Paluch
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  8. Małgorzata Kowalska
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  9. Zbigniew Wygoda
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Correspondence to Jarosław Markowski.

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Markowski, J., Tyszkiewicz, T., Jarząb, M. et al. Metal-proteinase ADAM12, kinesin 14 and checkpoint suppressor 1 as new molecular markers of laryngeal carcinoma . Eur Arch Otorhinolaryngol 266, 1501–1507 (2009). https://doi.org/10.1007/s00405-009-1019-3

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  • DOI: https://doi.org/10.1007/s00405-009-1019-3

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