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Creating artificial perichondrium by polymer complex membrane macroencapsulation: immune protection and stabilization of subcutaneously transplanted tissue-engineered cartilage

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Abstract

Functional organ or tissue failure is one of the most frequent, devastating and costly problems in modern health care. The field of tissue engineering has tremendous potential for developing new functional tissue. In reconstructive surgery, cartilage engineering could be a serious alternative to the established method of autologous cartilage transplantation. Recent studies demonstrate cartilage engineering by subcutaneous implantation of chondrocyte-seeded PGA/PLA-fibrin glue scaffolds in the backs of nude mice. In both autologous cartilage transplantation and cartilage engineering, the host immune response affects transplant integrity and cartilage morphology to an unforeseeable extent. To investigate whether polyelectrolyte complex (PEC) membranes can prevent rejection of cartilage transplants without neglecting tissue metabolism, tissue-engineered cartilage encapsulated with a PEC membrane was subcutaneously implanted in the backs of nude mice. Non-encapsulated tissue-engineered cartilage was used for the control group. Histochemistry and scanning electron microscopy were performed 4 and 12 weeks after implantation. There was no interaction between the host and the implant with an intact PEC membrane. With protection by PEC encapsulation, implanted tissue-engineered cartilage showed no signs of degeneration and had a significantly weaker cellular immune response than without it. Thus, PEC membrane encapsulation appears to be a novel approach for protecting cartilage implants from host immune response after autologous transplantation.

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Abbreviations

FCS:

fetal calf serum

IL-1:

interleukin-1

NaCs:

sodium cellulose sulfate

NO:

nitrogen monoxide

PEC:

polyelectrolyte complex

PDADMAC:

polydiallyl dimethyl ammonium chloride

PGA/PLA:

polyglycolic acid/ polylactide acid

PGE 2:

prostaglandin E 2

TNF-α:

tumor necrosis factor-α

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Acknowledgements

This study was supported in part by funding of the University Hospital Charité and the German Research Foundation (DFG, Ha 2729/1-2).

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Authors and Affiliations

  1. Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Charité, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany

    Andreas Haisch, Andreas Gröger, Christopher Gebert, Sergije Jovanovic & Benedikt Sedlmaier

  2. Department of Pathology, University Medical Center Charité, Campus Benjamin Franklin, Berlin, Germany

    Korinna Leder

  3. Department of Otorhinolaryngology and Head and Neck Surgery, St. Elisabeth Hospital of the Ruhr University of Bochum, Bleichstrasse 15, 44787, Bochum, Germany

    Jörg Ebmeyer & Holger Sudhoff

  4. Interdisciplinary Tissue Engineering Laboratory, Tucholskystrasse 2, 10117, Berlin, Germany

    Michael Sittinger

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  1. Andreas Haisch
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  2. Andreas Gröger
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  3. Christopher Gebert
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  4. Korinna Leder
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  5. Jörg Ebmeyer
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  7. Sergije Jovanovic
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  8. Benedikt Sedlmaier
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  9. Michael Sittinger
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Correspondence to Andreas Haisch.

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Haisch, A., Gröger, A., Gebert, C. et al. Creating artificial perichondrium by polymer complex membrane macroencapsulation: immune protection and stabilization of subcutaneously transplanted tissue-engineered cartilage. Eur Arch Otorhinolaryngol 262, 338–344 (2005). https://doi.org/10.1007/s00405-004-0783-3

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  • DOI: https://doi.org/10.1007/s00405-004-0783-3

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