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Comparison of DNA damage in granulosa cells of women undergoing controlled ovarian stimulation in in vitro fertilization protocols with the recombinant human follicle-stimulating hormones Corneumon®, Gonal-F®, Pergoveris® and Puregon®: a randomized trial

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To compare the DNA damage in granulosa cells (GCs) of women undergoing ovarian-stimulated cycles with four widely used recombinant human follicle-stimulating hormones (rhFSH) in in vitro fertilization (IVF) protocols (Corneumon®, Gonal-F®, Pergoveris® and Puregon®).

Methods

A randomized trial was carried out at a Mexican hospital. GCs were isolated from 18 women with infertility undergoing assisted reproductive techniques (ART). Four controlled ovarian stimulation (COS) protocols including Corneumon®, Gonal-F®, Pergoveris® or Puregon® were used. GCs DNA damage was assessed by the Comet assay. Two parameters were measured: comet tail length (CTL), and Olive tail moment (OTM, the percentage of DNA in the tail multiplied by the distance between the center of the tail and head).

Results

Use of the different hrFSH in COS caused variable and statistically significant levels of DNA damage in GCs of infertile women. CTL was similar in the Corneumon® and Pergoveris® groups (mean values of 48.73 and 55.18, respectively) and Corneumon® CTL was significantly lower compared to the Gonal-F® and Puregon® groups (mean values of 61.98 and 91.17, respectively). Mean OTM values were significantly lower in Corneumon® and Pergoveris® groups, compared to Gonal-F® and Puregon® groups (25.59, 27.35, 34.76, and 47.27, respectively).

Conclusion

Use of Corneumon® and Pergoveris® in COS caused statistically significantly lower levels of DNA damage in GCs of infertile women undergoing ART, which could potentially correlate with better reproductive outcomes.

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Acknowledgements

This research was supported by the Metropolitan Autonomous University, Iztapalapa campus of Mexico City. X.A.S. was recipient of a scholarship from the Mexican Council for Science and Technology (CONACYT).

Funding

This work was supported by the Metropolitan Autonomous University, Iztapalapa campus, Mexico City.

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Author notes

  1. Ximena Alcalá-Sánchez and Pedro Cuapio-Padilla contributed equally to this article as first authors.

Authors and Affiliations

  1. Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico

    Ximena Alcalá-Sánchez, Mario Teteltitla, Iván Bahena, Miguel Betancourt, Eduardo Casas, Alma López & Edmundo Bonilla

  2. Master’s Degree Program in Animal Reproduction Biology, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico

    Ximena Alcalá-Sánchez

  3. HISPAREP Assisted Reproduction Clinic, Hospital Español, 11520 CDMX, Mexico City, Mexico

    Pedro Cuapio-Padilla, Carlos Salazar-López & Ricardo Rodríguez

  4. Department of Biology of Reproduction, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico

    Fahiel Casillas

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Contributions

X Alcalá-Sánchez, P Cuapio-Padilla, C Salazar-López and E Bonilla: protocol/project development, data collection, data management, data analysis, manuscript writing/editing. R Rodríguez, M Teteltitla, I Bahena, M Betancourt, E Casas, F Casillas and A López: data management, data analysis, manuscript writing/editing. X Alcalá-Sánchez: protocol/project development, data collection, data management, data analysis, manuscript writing/editing. P Cuapio-Padilla: protocol/project development, data collection, data management, data analysis, manuscript writing/editing. C Salazar-López: protocol/project development, data collection, data management, data analysis, manuscript writing/editing. R Rodríguez: data management, data analysis, manuscript writing/editing. M Teteltitla: data management, data analysis, manuscript writing/editing. I Bahena: data management, data analysis, manuscript writing/editing. M Betancourt: data management, data analysis, manuscript writing/editing. E Casas: data management, data analysis, manuscript writing/editing. F Casillas: data management, data analysis, manuscript writing/editing. A López: data management, data analysis, manuscript writing/editing. E Bonilla: protocol/project development, data collection, data management, data analysis, manuscript writing/editing.

Corresponding author

Correspondence to Edmundo Bonilla.

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Ethics approval and Consent to participate

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Institutional Review Board of the HISPAREP Assisted Reproduction Clinic (HISPCHB21-01). Informed consent was obtained from all individual participants included in the study.

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The manuscript does not contain any individual person’s data in any form (including any individual details, images or videos).

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Alcalá-Sánchez, X., Cuapio-Padilla, P., Salazar-López, C. et al. Comparison of DNA damage in granulosa cells of women undergoing controlled ovarian stimulation in in vitro fertilization protocols with the recombinant human follicle-stimulating hormones Corneumon®, Gonal-F®, Pergoveris® and Puregon®: a randomized trial. Arch Gynecol Obstet 309, 2107–2114 (2024). https://doi.org/10.1007/s00404-024-07392-7

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