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Smooth endoplasmic reticulum cluster presence does not affect embryo ploidy

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

We examined the impacts of the smooth endoplasmic reticulum cluster (sERC) presence on embryonic development and blastocyst ploidy.

Methods

Patients who underwent oocyte retrieval from January 2019 to November 2021 were included in the study. We classified the oocytes into three groups: normal oocytes in the sERC ( −) cycle, normal oocytes in the sERC ( +) cycle, and sERC ( +) oocytes. Next, the levels of serum estradiol, progesterone, anti-Mullerian hormone, follicle-stimulating hormone, and human menopausal gonadotropin were compared between the groups. Moreover, fertilization, degeneration, and abnormal fertilization rates were compared between groups. To investigate developmental outcomes, the blastocyst and good-quality blastocyst rates after intracytoplasmic sperm injection were compared. The quality of the transferred blastocysts was evaluated at follow-up. Additionally, embryos were submitted for next-generation sequencing analysis to examine the effect of sERC presence on ploidy.

Results

The sERC ( +) group had significantly higher serum estradiol, serum progesterone, and serum anti-Mullerian hormone concentrations compared to those in the sERC ( −) group (P < 0.01). The abnormal fertilization rate was higher in the sERC ( +) cycle–sERC ( +) oocyte group (16.1%; 37/230) than in the sERC ( +) cycle–normal oocyte (6.2%; 63/971) and sERC ( −) cycle–normal oocyte groups (7.1%; 174/2467) (P < 0.01). After embryo transfer, nine women gave birth, and no confirmed congenital anomalies were observed. There was no significant difference in ploidy between the sERC ( +) and sERC ( −) groups.

Conclusion

The occurrence rates of embryos with euploidy were similar between the sERC ( +) and sERC ( −) groups.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

No funds, grants, or other support was received for this work.

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Authors and Affiliations

  1. Takeuchi Ladies Clinic/Center for Reproductive Medicine, 502-2 Higashimochida, Aira-shi, Kagoshima, 899-5421, Japan

    Yamato Mizobe, Yukari Kuwatsuru, Yuko Kuroki, Yumiko Fukumoto, Mari Tokudome, Harue Moewaki, Marina Tabira, Tokiko Iwakawa & Kazuhiro Takeuchi

Authors
  1. Yamato Mizobe
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  2. Yukari Kuwatsuru
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  3. Yuko Kuroki
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  4. Yumiko Fukumoto
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  5. Mari Tokudome
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  6. Harue Moewaki
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  7. Marina Tabira
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  8. Tokiko Iwakawa
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  9. Kazuhiro Takeuchi
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by YM, MT, and YK. The first draft of the manuscript was written by YM and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yamato Mizobe.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

The study was performed with the approval of the institutional review board of the Takeuchi Ladies Clinic (approval number 22–1). Opt-out information was posted on a hospital bulletin board.

Human and animal rights

All procedures were performed in accordance with the ethical standard of the responsible committees on human experimentation (institutional and national) and with the Helsinki Declaration of 1964 and its later amendments.

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Informed consent was obtained from all patients in the study.

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Mizobe, Y., Kuwatsuru, Y., Kuroki, Y. et al. Smooth endoplasmic reticulum cluster presence does not affect embryo ploidy. Arch Gynecol Obstet 307, 1607–1612 (2023). https://doi.org/10.1007/s00404-023-06969-y

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  • DOI: https://doi.org/10.1007/s00404-023-06969-y

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