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Annexin A5 suppression promotes the progression of cervical cancer

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Background

Cervical cancer is a common malignant gynecological disease that threatens the health of women all over the world. The abnormal expression of Annexin A5 (ANXA5) is closely related to the biological behavior of various malignant tumors, however, the relationship between ANXA5 and cervical cancer is still unclear. Therefore, the effects of low expression of ANXA5 on the proliferation, apoptosis, migration and invasion of cervical cancer cells (HeLa) and its related mechanism were explored.

Methods

The cells were divided into three groups: ANXA5-si group, negative control group and blank group. RNA interference was used to suppress ANXA5 expression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, flow cytometry and propidium iodide (PI) staining, wound healing assay and transwell assay were employed to detect cell proliferation, apoptosis, migration and invasion respectively. Meanwhile, gene expression was detected by qPCR and Western blotting.

Results

ANXA5 suppression lead to the increase of proliferation, migration, invasion and the decrease of apoptosis of cervical cancer HeLa cells. Furthermore, the expression of both pPI3K and pAkt increased.

Conclusion

ANXA5 might inhibit Hela cells proliferation and metastasis by regulating PI3K/Akt signal pathway.

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Funding

The present study was supported by the Key Subjects in University of Hebei Province (Grant no. 2013-4) and by the Education Department of Hebei Province (Grant no. Z2014026).

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Authors and Affiliations

  1. Chengde Medical University, Chengde, 067000, Hebei, The People’s Republic of China

    Xiaojie Wang, Yarui Dai, Jialu Zhang & Xin Li

Authors
  1. Xiaojie Wang
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  2. Yarui Dai
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  3. Jialu Zhang
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  4. Xin Li
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Contributions

XW: experiments operation; YD: data collection, data analysis, manuscript writing; JZ: data collection, manuscript writing; XL: project development, manuscript editing.

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Correspondence to Xin Li.

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Wang, X., Dai, Y., Zhang, J. et al. Annexin A5 suppression promotes the progression of cervical cancer. Arch Gynecol Obstet 307, 937–943 (2023). https://doi.org/10.1007/s00404-022-06524-1

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  • DOI: https://doi.org/10.1007/s00404-022-06524-1

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