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Dose-dependent effects of ghrelin and aberrant anti-Mullerian hormone levels in the prevention of ovarian damage caused by cisplatin in Wistar-albino rats

Abstract

Purpose

Ghrelin has previously been proven to have anti-inflammatory and antioxidant properties in preventing cisplatin-induced ovarian damage. The aim of this study was to evaluate the potential effects of this hormone in preventing this damage in rats using histopathological and biochemical methods.

Methods

Twenty-eight Wistar-albino rats were randomly divided into four groups. While no drug was given to Group 1 (sham group), acylated ghrelin was intraperitoneally administered to Group 2 at 0.5 nmol/kg and Group 3 at 2 nmol/kg for 21 days. Group 4 received only saline solution. On the 15th day, a single dose of 5 mg/kg cisplatin was intraperitoneally administered to each rat in Groups 2, 3 and 4. Serum anti-Mullerian hormone (AMH) values were measured on days 0, 15 and 21. Then, laparotomy and bilateral oophorectomy were performed, and the ovaries were histopathologically examined.

Results

The number of primordial and primary follicles was significantly higher in Group 3 than in the saline solution + cisplatin group. In Group 4, cisplatin caused significantly higher follicle damage in the primordial, primary and secondary phases compared to the sham group. The AMH level of the SF + cisplatin group was significantly lower than that of the sham group and the high-dose ghrelin + cisplatin group, and the AMH level of the sham group was significantly higher than that of the low-dose ghrelin + cisplatin group.

Conclusion

High-dose ghrelin was effective in preventing cisplatin-induced ovarian damage by preserving the number of primordial and primary follicles. Larger randomized studies are needed to determine the optimal dosage and duration of ghrelin.

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Availability of data and material

The data are available.

Code availability

Not applicable.

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Funding

The study was not funded by a grant.

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Contributions

EOT: protocol/project development, data analysis, manuscript writing/editing. MG: Data analysis, manuscript writing/editing. MSK: data collection or management. AM: data collection or management. DY: data collection or management. SH: data collection or management. MÇ: data collection or management. YÜ: protocol/project development, data analysis.

Corresponding author

Correspondence to Murat Gözüküçük.

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The authors reported no conflict of interest.

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The study was approved by the Local Ethics Committee of Health Sciences University Ankara Health Research Application Center Animal Experiments (2018/47-534) was conducted in accordance with the ethical rules of the committee and standards proposed by the Council of Europe (European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes) (ETS 123).

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Tutar, E.O., Gözüküçük, M., Kaya, M.S. et al. Dose-dependent effects of ghrelin and aberrant anti-Mullerian hormone levels in the prevention of ovarian damage caused by cisplatin in Wistar-albino rats. Arch Gynecol Obstet (2021). https://doi.org/10.1007/s00404-021-06292-4

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Keywords

  • Ghrelin
  • Cisplatin
  • AMH
  • Ovarian follicles
  • Fertility preservation