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Evaluation of dynamic thiol–disulfide balance in preinvasive lesions of the cervix

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

This study aimed to determine the potential clinical use of dynamic thiol–disulfide balance in cases with preinvasive lesions of the cervix.

Methods

One hundred and sixteen patients with high-grade squamous intraepithelial lesion, 100 patients with low-grade squamous intraepithelial lesion, and 110 healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol–disulfide parameters. The ischemia-modified albumin, total oxidant–antioxidant capacity, and oxidative stress index of the retrieved cases were further analyzed.

Results

Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p: 0.004 and 0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p: 0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p: 0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p: 0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r: 0.420, p < 0.001).

Conclusion

The analysis of dynamic thiol–disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus-related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol–disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

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Funding

We did not have any financial support. These findings have not been presented anywhere.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Muğla Sıtkı Koçman University, Kötekli district No: 48, 48000, Muğla, Turkey

    Burak Sezgin & Fatih Pirinççi

  2. Department of Obstetrics and Gynecology, Private Adatıp Sakarya Hospital, İstiklal district, Şehit Mehmet Karabaşoğlu Street, No:67, Serdivan, Sakarya, Turkey

    Aysun Camuzcuoğlu & Hakan Camuzcuoğlu

  3. Department of Obstetrics and Gynecology, Malatya Education and Research Hospital, Özalper district Turgut Özal street No:4 44330, Malatya, Turkey

    E. Adeviye Şahin

  4. Department of Clinical Biochemistry, Faculty of Medicine, Yıldırım Beyazit University, Çankırı street Çiçek district No:3 Altındağ 06000 Ulus, Ankara, Turkey

    Özcan Erel & Salim Neşelioğlu

  5. Department of Gynecologic Oncology, Private Adatıp Sakarya Hospital, İstiklal district, Şehit Mehmet Karabaşoğlu Street, No:67, Serdivan, Sakarya, Turkey

    Hakan Camuzcuoğlu

Authors
  1. Burak Sezgin
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  2. Fatih Pirinççi
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  3. Aysun Camuzcuoğlu
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  4. E. Adeviye Şahin
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  5. Özcan Erel
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  6. Salim Neşelioğlu
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  7. Hakan Camuzcuoğlu
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Contributions

BS: project administration, writing—reviewing and editing; FP: data curation, methodology, and investigation; AC and EAŞ: visualization, writing—original draft preparation; ÖE and SN: formal analysis; HC: conceptualization, supervision, and validation.

Corresponding author

Correspondence to Burak Sezgin.

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Conflict of interest

B.Sezgin, Fatih Pirinççi, A.Camuzcuoğlu, Eda Adeviye Şahin, Özcan Erel, Salim Neşelioğlu and H.Camuzcuoğlu declare that they have no conflict of interests.

Ethical approval

Institutional review board approval for this research was obtained from Mugla Sitki Kocman University Medical Ethics Committee (Approval date and number: 19/09/2018-03). The trial was registered at ClinicalTrials.gov (ID: NCT04177641). An informed consent following the declaration of Helsinki was signed and obtained from the participants.

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Sezgin, B., Pirinççi, F., Camuzcuoğlu, A. et al. Evaluation of dynamic thiol–disulfide balance in preinvasive lesions of the cervix. Arch Gynecol Obstet 305, 617–623 (2022). https://doi.org/10.1007/s00404-021-06252-y

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  • DOI: https://doi.org/10.1007/s00404-021-06252-y

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