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Prenatal sonographic and cytogenetic/molecular findings of 22q11.2 microdeletion syndrome in 48 confirmed cases in a single tertiary center

  • Maternal-Fetal Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

We aimed to present the fetal ultrasound, cytogenetic/molecular testing and postmortem or postnatal clinical findings of cases with 22q11.2DS diagnosed prenatally.

Materials and methods

A retrospective medical record review of 48 prenatal cases diagnosed with 22q11.2DS were evaluated in our institution. Detailed ultrasound examination was performed on all fetuses. Postmortem and postnatal examinations were evaluated. The microdeletions were detected by karyotyping or microarray, then confirmed by FISH. Descriptive statistical analysis was performed.

Results

Demographic data of 48 prenatal cases including 46 singletons and 1 dichorionic diamniotic twin pregnancy were evaluated. The most common extracardiac anomaly was skeletal system anomalies (25%), in which PEV was the most frequent one (20.8%). Polyhydramnios rate was detected as 31%, in 6.6% as an isolated finding. Microdeletion has been detected by karyotyping in 13 cases (13/47, 27.7%) (including 2 unbalanced translocations), by FISH in 28 cases (28/48, 58.3%), by microarray/a-CGH testing in 7 cases. Microarray analysis showed that in one case with unbalanced translocation had two consecutive deletions; one was proximal and other one distal to critical region and not encompassing TBX1 gene but CRKL and LZTR1 genes.

Conclusion

The current study demonstrates the whole spectrum of atypical phenotypic and genotypic variations of 22q11.2DS in the largest prenatal case series reported to date. Therefore, differential diagnosis should be considered not solely in CHD, but also in the presence of isolated clubfeet and polyhydramnios. Establishing the diagnosis in the prenatal period may allow a postnatal multidisciplinary approach, as well as affect the actual prevalence of the disease.

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Availability of data and material

Data will be made available by the authors upon request.

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Authors and Affiliations

  1. Division of Perinatology, Department of Obstetrics and Gynecology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Tugba Sarac Sivrikoz, Recep Has, Ibrahim Halil Kalelioglu & Atil Yuksel

  2. Department of Medical Genetics, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Seher Basaran, Birsen Karaman & Umut Altunoglu

  3. PREMED, Center for Genetic Diagnosis and Research, Mecidiyekoy, Istanbul, Turkey

    Seher Basaran & Melike Kirgiz

  4. Department of Pediatric Basic Science, Child Health Institute, Istanbul University, Istanbul, Turkey

    Birsen Karaman

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  1. Tugba Sarac Sivrikoz
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by TSS, UA, MK and BK. The manuscript was written by TSS, AY, RH and SB. TSS, IHK and RH analyzed and interpreted of the data.

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Correspondence to Tugba Sarac Sivrikoz.

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Sarac Sivrikoz, T., Basaran, S., Has, R. et al. Prenatal sonographic and cytogenetic/molecular findings of 22q11.2 microdeletion syndrome in 48 confirmed cases in a single tertiary center. Arch Gynecol Obstet 305, 323–342 (2022). https://doi.org/10.1007/s00404-021-06125-4

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