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The inhibition of bone morphogenetic protein 1 attenuates endometriosis lesions in vivo and in vitro

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To investigate the potential role of Bone morphogenetic protein 1 (BMP1) in endometriosis lesions.

Methods

Endometriosis model in mice was established. The expression of BMP1-3 expression in mice of endometriosis lesions was evaluated. The effect of the treatment with anti-BMP1 antibodies on the expression of MMP2, MMP9, TGF-β, IL-17, IL-1β, Col1a1 and Col1a2 levels in mice was evaluated. In endometriosis cell model, the expression of IL-17, IL-1β, MMP2 and MMP9 levels and MIF, YWHAZ, β-catenin and CAP39 mRNA levels was also detected.

Results

The expression of BMP1-3 expression was upregulated in mice of endometriosis lesions (p < 0.01). Treatment with anti-BMP1 antibodies dose-dependently reduced MMP2, MMP9, TGF-β, IL-17, IL-1β, Col1a1 and Col1a2 levels in mice (p < 0.01). Treatment with anti-BMP1 antibodies suppressed TGF-β/PI3K/Akt signaling pathway. In vitro cell, si-BMP1 suppressed TGF-β/PI3K/Akt signaling pathway.

Conclusion

The data support the hypothesis that the inhibition of BMP1 is involved in the pathogenesis of endometriosis lesions.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shenzhen University General Hospital, Shenzhen, China

    Xinyue Han & Feifei Hu

  2. Department of Obstetrics and Gynecology, Baotou City Central Hospital, Baotou, China

    Fang Chen

  3. Department of Neurology, The University of Hong Kong-Shenzhen Hospital, 1 Haiyuan 1st Road, Futian District, Shenzhen City, 518000, China

    Wei Wang

Authors
  1. Xinyue Han
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  2. Feifei Hu
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  3. Fang Chen
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  4. Wei Wang
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Contributions

WW carried out the study concepts; XH, WW were dedicated to the study design, literature research and manuscript review; XH was involved in the definition of intellectual content, experimental studies, data analysis, manuscript preparation and manuscript editing; XH and FH were dedicated to clinical studies; XH, FH and FC carried out the data acquisition; FH and FC were involved in the statistical analysis. All authors have read and approved this article.

Corresponding author

Correspondence to Wei Wang.

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The authors declare that they have no competing interest.

Ethical statement

This study was carried out in accordance with recommendations of the regulations for animal experimentation in Hyogo College of Medicine of The University of Hong Kong-Shenzhen Hospital.

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Han, X., Hu, F., Chen, F. et al. The inhibition of bone morphogenetic protein 1 attenuates endometriosis lesions in vivo and in vitro. Arch Gynecol Obstet 302, 415–422 (2020). https://doi.org/10.1007/s00404-020-05612-4

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  • DOI: https://doi.org/10.1007/s00404-020-05612-4

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