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Does timing in ICSI cycle affect oocyte quality and reproductive outcomes? A prospective study

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

A Correction to this article was published on 29 May 2020

This article has been updated

Abstract

Purpose

To evaluate the association of time intervals between various steps of the intracytoplasmic sperm injection (ICSI) cycle with oocyte quality and reproductive outcomes.

Methods

We conducted a prospective study among patients undergoing ICSI cycles in an academic hospital between May 2017 and January 2019. The time intervals between the various steps of cycles were recorded. The ICSI cycles were categorized according to the different time intervals; human chorionic gonadotropin (hCG) injection to oocyte pick up (hCG-OPU) (≤ 36 h and > 36 h), OPU-denudation (≤ 2 h and > 2 h), and denudation-ICSI (≤ 2 h and > 2 h). The main outcome measures were oocyte dysmorphisms, fertilization, cleavage, biochemical, and clinical pregnancy rates.

Results

A total of 613 ICSI cycles using fresh autologous oocytes were included in this study. After adjusting for confounders, the hCG–OPU interval was associated with the presence of cytoplasmic granulation, inclusion body, and also the total number of morphologically abnormal premature oocytes in the cycle (P = 0.02, P = 0.04, P = 0.008, respectively). OPU-denudation interval was associated with cytoplasmic granulation and extended perivitelline space of the oocytes (P = 0.006 and P = 0.03, respectively). The denudation-ICSI interval was only associated with cytoplasmic granulation (P = 0.01). However, hCG–OPU, OPU–denudation, and denudation–ICSI intervals were not significantly associated with fertilization, cleavage, biochemical, and clinical pregnancy rates.

Conclusions

All the studied time intervals between various steps of ICSI procedure could affect oocyte quality, but the oocyte dysmorphisms were mainly associated with hCG-OPU interval. However, the time intervals were not associated with fertilization, cleavage, and pregnancy outcomes.

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Change history

  • 29 May 2020

    In the original article published, the values given in the variables are incorrect.

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Acknowledgements

This study is financially supported by Men's health and Reproductive Health Research Center (MHRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran (grant number: 12102).

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Authors and Affiliations

  1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Elham Azizi & Hamid Nazarian

  2. Urology and Nephrology Research Center (UNRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Naji & Nasrin Borumandnia

  3. IVF Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Saghar Salehpour & Maryam karimi

  4. Men’s Health and Reproductive Health Research Center (MHRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zahra Shams Mofarahe

Authors
  1. Elham Azizi
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  2. Mohammad Naji
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  3. Hamid Nazarian
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  4. Saghar Salehpour
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  5. Maryam karimi
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  6. Nasrin Borumandnia
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  7. Zahra Shams Mofarahe
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Contributions

EA: protocol development, data collection, data analysis, and manuscript writing; MN: data collection, data analysis, and manuscript writing; HN: project development and data collection; SS: project development and data collection; MK: data collection and data analysis; NB: data collection and data analysis; ZSM: project development, data analysis, and manuscript editing.

Corresponding author

Correspondence to Zahra Shams Mofarahe.

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Conflict of interest

The authors of the current paper declare no conflict of interest regarding all periods of working—neither financial support nor personal relationship. Elham Azizi declares that she has no conflict of interest. Mohammad Naji declares that he has no conflict of interest. Hamid Nazarian declares that he has no conflict of interest. Saghar Salehpour declares that she has no conflict of interest. Maryam Karimi declares that she has no conflict of interest. Nasrin Borumandnia declares that she has no conflict of interest. Zahra Shams Mofarahe declares that she has no conflict of interest.

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Informed consent was obtained from all participants.

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The original version of this article was revised due to the incorrect variable value given in the tables.

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Azizi, E., Naji, M., Nazarian, H. et al. Does timing in ICSI cycle affect oocyte quality and reproductive outcomes? A prospective study. Arch Gynecol Obstet 302, 505–513 (2020). https://doi.org/10.1007/s00404-020-05555-w

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  • DOI: https://doi.org/10.1007/s00404-020-05555-w

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