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Decreased expression of progesterone receptor membrane component 1 in fetal membranes with chorioamnionitis among women with preterm birth

  • Maternal-Fetal Medicine
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Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Progesterone receptor membrane component 1 (PGRMC1) have anti-inflammatory and anti-apoptotic properties. This study aimed to determine the expression of PGRMC1 in fetal membranes among women with preterm labor (PTL), preterm premature rupture of membranes (PPROM), and acute histologic chorioamnionitis (HCA) during preterm birth.

Methods

Full thickness fetal membranes were obtained from women with gestational age-matched (32–34 weeks of gestational age), and categorized as PTL without HCA (PTL, n = 10), PPROM without HCA (PPROM, n = 10), PPROM with HCA (HCA, n = 10), and term without labor and HCA (term birth (TB), n = 9). The expression of PGRMC1 was assessed using western blot and Immunohistochemistry (IHC). As CD14 is a component of the innate immune system during inflammation, CD14 was used as inflammatory indicator. Nonparametric statistics were used for analysis.

Results

PGRMC1 expression for all of preterm birth was lower than in TB (P = 0.01). In HCA, PGRMC1 expression was significantly decreased compared to that in PTL and PPROM (P = 0.006. P = 0.001, respectively). PGRMC1 expression in PPROM was higher than that in PTL (P = 0.002). There was a negative correlation between PGRMC1 and CD 14/β-actin ratio (r = − 0.518; P = 0.002). IHC showed that PGRMC1 was predominant in the cytoplasm of cells, these results were consistent with those of the western blot analysis.

Conclusion

Preterm birth with PTL, PPROM, and especially HCA is associated with a decreased PGRMC1 in fetal membranes and inversely associated with increased CD 14.

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Acknowledgements

We would like to thank Ji Young Seo for laboratory work.

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government [No. 2017R1D1A1B03029882].

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Inha University College of Medicine, Inha University Hospital, 27, Inhang-Ro, Jung-Gu, Incheon, 22332, South Korea

    Soo Ran Choi, Hyung Eun Choi, Eunae Jo, Hwa Yeon Choi, Sukyung Jung, Shina Jang & Sung Ook Hwang

  2. Department of Pathology, Inha University College of Medicine, Inha University Hospital, Incheon, South Korea

    Suk Jin Choi

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  1. Soo Ran Choi
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  2. Hyung Eun Choi
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Contributions

SRC: Protocol/Project development, Data collection and analysis, Manuscript writing. HEC, EJ, HYC, SJ and SJ: collect the fetal membranes. SJC: examin histologic findings and IHC staining of fetal membranes. SOH: Manuscript editing.

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Correspondence to Soo Ran Choi.

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Choi, S.R., Choi, H.E., Jo, E. et al. Decreased expression of progesterone receptor membrane component 1 in fetal membranes with chorioamnionitis among women with preterm birth. Arch Gynecol Obstet 301, 949–954 (2020). https://doi.org/10.1007/s00404-020-05476-8

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  • DOI: https://doi.org/10.1007/s00404-020-05476-8

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