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Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

It is known that menopausal osteoporosis (MOP) is the most typical form of osteoporosis, which is characterized by low bone mass and microstructure damage of the bone tissue, leading to increased bone fragility and risk of fracture. This study aimed to evaluate the protective effects of Oviductus Ranae protein hydrolyzate (ORPH) on the MOP in vivo.

Methods

Osteoporosis model was induced by ovariectomy, treated with ORPH 150 or 75 mg kg−1. Body weight and bone mineral density (BMD) of rats were measured at the beginning and the end of the experiment, and femoral maximum load was determined immediately after killing. The expression levels of alkaline phosphatase (ALP), Smad4, tartrate acid phosphatase (TRAP), BMP2, Runx2, CPB, ColI and osteocalcin were examined by RT-PCR or western-blotting. HE staining was used to observe the pathological changes in the femurs. Immunohistochemistry was used to detect the expression of ALP and BMP2. All data were analyzed by SPSS 13.0.

Results

The results revealed that ORPH had no effect on the weight of normal and osteoporotic rats. ORPH could significantly improve the femur BMD and increase the maximum load of the osteoporotic rats. ORPH could significantly upregulate the expression level of bone formation makers, ALP, osteocalcin, ColI, and Runx2, and downregulate the expression level of bone resorption marker, TRAP. In the ORPH group, the expression levels of BMP2, Smad4, and CPB of key proteins in the TGFβ/BMP2 signaling pathway were significantly upregulated. In addition, immunohistochemistry showed that ALP and BMP2 expression in femurs of the ORPH group was stranger. H&E staining showed that ORPH (150 mg kg−1) significantly increased the thickness of trabeculae and decreased fracture risk.

Conclusion

Collectively, ORPH plays a role in the prevention and treatment of osteoporosis, which may be a potential anti-osteoporosis drug.

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Acknowledgements

This work was funded by Jilin Province Traditional Chinese Medicine Administration and Jilin Province Department of Education.

Funding

This study was funded by the Jilin Province Traditional Chinese Medicine Science and Technology Project (2017002, 2018024); the Jilin Provincial Department of Education Science Research Project (JJKH20181266KJ); the Jilin province science and technology backbone training project for health and hygiene (2018Q043).

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Authors and Affiliations

  1. Changchun University of Chinese Medicine, Boshuo Road, No. 1035, Jing Yue Development Zone, Changchun, 130117, China

    Xiaohua Li, Qing Yang, Yinqing Li, Na Li, Xiaozheng Shi, Dong Han, Yiping Li, Xiaowei Huang, Peng Yu & Xiaobo Qu

  2. Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130021, China

    Xin Sui

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  1. Xiaohua Li
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  2. Xin Sui
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  3. Qing Yang
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  5. Na Li
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  11. Xiaobo Qu
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Contributions

XL, XQ and NL conceived and designed the study. XL, XS, QY, and XS performed the experiments. YL provided the Oviductus Ranae. XL wrote the paper. DH, YL, XH, PY and XQ reviewed and edited the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Xiaobo Qu.

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Conflict of interest

The authors declare no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Li, X., Sui, X., Yang, Q. et al. Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling. Arch Gynecol Obstet 299, 873–882 (2019). https://doi.org/10.1007/s00404-018-5033-9

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  • DOI: https://doi.org/10.1007/s00404-018-5033-9

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