Expression of semaphorin class 3 is higher in the proliferative phase on the human endometrium
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The semaphorins are related to angiogenesis and cell proliferation depending on the tissue. The purpose of this study was to assess gene expression of class 3 semaphorin (SEMA3A-F) and protein expression of semaphorin 3A (SEMA3A) within human endometrium throughout the menstrual cycle.
Gene expression of SEMA3A-F was analyzed by real-time PCR (qRT-PCR) and protein expression of SEMA3A was analyzed by ELISA in endometrial biopsies in the proliferative and secretory phase of the menstrual cycle.
Gene expression of SEMA3A, SEMA3C, SEMA3D, and SEMA3E was statistically significant decreased in secretory compared to proliferative phase endometrium (p < 0.05). Accordingly, SEMA3A protein expression in the secretory phase was lower than protein expression in proliferative phase endometrium (p ≤ 0.05).
SEMA3A, 3C, 3D, and 3E are possibly related to cell proliferation in the endometrium, being more expressed in the proliferative phase of the cycle. This finding may stimulate studies of class 3 semaphorins as a possible target for treatment of endometrial pathologies.
KeywordsEndometrium Semaphorin Menstrual cycle SEMA3
Gustavo Dias Ferreira was scholarship recipient from CAPES—Process Number 18471-12-9.
GDF project development, data management, data analysis, and manuscript writing. EC and AG project development, data analysis, and manuscript editing. JJ project development and data management. TS project development, data collection, and manuscript editing.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This project was approved by the Ethics Committee of the Medical Faculty of the Heidelberg University (Number 239-05).
- 13.Toyofuku T, Zhang H, Kumanogoh A, Takegahara N, Suto F, Kamei J et al (2004) Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2. Genes Dev 18(4):435–447CrossRefPubMedPubMedCentralGoogle Scholar
- 27.Regano D, Visintin A, Clapero F, Bussolino F, Valdembri D, Maione F et al (2017) Sema3F (semaphorin 3F) selectively drives an extraembryonic proangiogenic program. Arterioscler Thromb Vasc Biol 37(9):1710–1721. https://doi.org/10.1161/ATVBAHA.117.308226 (Epub 2017 Jul 20) CrossRefPubMedPubMedCentralGoogle Scholar