Archives of Gynecology and Obstetrics

, Volume 297, Issue 5, pp 1271–1275 | Cite as

Discrepancy between colposcopy, punch biopsy and final histology of cone specimen: a prospective study

  • Stamatios Petousis
  • Panagiotis Christidis
  • Chrysoula Margioula-Siarkou
  • Nikolaos Sparangis
  • Apostolos Athanasiadis
  • Ioannis Kalogiannidis
Gynecologic Oncology



Τo estimate the discrepancy rate between colposcopy, punch biopsy histology and surgical specimen histology as well as the positive (PPV) and negative predictive value (NPV) of colposcopic diagnosis for high-grade squamous intraepithelial lesions (HGSIL).


A prospective study was conducted during the period of 2012–2016. Αll cases in which surgical treatment had been applied and histopathological diagnosis of those surgical specimens was available were included. Cases in which ablation was performed and cases with incomplete data or conservative approach were excluded. Primary outcome was the agreement rate between histologic diagnosis of surgical specimen, histologic diagnosis of punch biopsy and colposcopic diagnosis according to REID Colposcopic Index. PPV and NPV of colposcopy and biopsy to diagnose HGSIL were also assessed.


Τhere were 120 cases meeting our inclusion criteria, while biopsy was obtained in 104 cases. Mean age of women was 32.7 ± 9.0. Colposcopic diagnosis was CIN2 in 65 cases, CIN3 in 11 cases, CIN1 or less in 44 cases. Τhe level of agreement was fair between colposcopy-surgical specimen histology (κ value 0.443), fair between colposcopy—punch biopsy (κ value 0.34) and moderate between punch biopsy—cone specimen histology (κ value 0.443). PPV of colposcopy to detect HGSIL was 72.3%, while NPV was only 47.7%.


Punch biopsy and surgical specimen histology present the highest agreement between the different diagnostic procedures. Colposcopy presented satisfying PPV for HGSIL cases, but its NPV was poor. In contrary, punch biopsy was characterized by both satisfying PPV and NPV for HGSIL cases.


Colposcopy Punch biopsy Predictive value Discrepancy HGSIL Intervention 


Author contributions

SP: protocol development, data collection, data analysis, manuscript writing. PC: data collection, data analysis, manuscript writing. CM-S: protocol development, data collection, data analysis, manuscript writing. NS: data collection, data analysis, manuscript writing. AA: project supervision, manuscript reviewing. IK: project supervision, manuscript reviewing.

Role of the funding source

The authors declare that they received no funding.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

IRB status

Institutional Review Board and Ethical Review Committee have given approval to the present study.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.3rd Department of Obstetrics and GynaecologyAristotle University of ThessalonikiThessalonikiGreece

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