Differential JNK, p38 and ERK response to renal injury in a rat model of acute pancreatitis in pregnancy
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The objective of this study was to determine the mechanism of acute renal injury (ARI) in acute necrotizing pancreatitis in late pregnancy (ANPIP).
Pregnant Sprague–Dawley rats in the third trimester were used for this study, and an ANPIP model was induced by injecting 5% sodium taurocholate into the biliary pancreatic duct. The rats were randomly divided into three groups: the normal, sham-operated (SO) and acute necrotizing pancreatitis (ANP) groups. Rats were killed at 3, 6, 12 h after the operation, and blood, pancreatic and renal tissue samples were harvested. Differences were detected in the physiology, pathology and cellular and molecular responses among the different groups.
Serum amylase, lipase, urea and Cr levels were increased in rats with ANPIP. Additionally, expression of phosphorylation p38 and JNK as well as TNF-α and NF-κB were increased in the renal tissues of rats with ANPIP. The expression of phosphorylation ERK was decreased in the renal tissues of rats with ANPIP.
Mitogen-activated protein kinases may play an important role in renal injury in rat models of ANPIP.
KeywordsAcute pancreatitis Pregnancy Renal injury MAPK
We would like to thank the National Natural Science Foundation of China (Grant no. 81370562) for providing funds for this project.
F-CM: project development, data collection, data analysis and manuscript writing. TZ: data analysis and manuscript writing. LZ: manuscript writing and manuscript review. QS: data analysis and manuscript editing. M-WX: data collection. Y-PH: data collection and manuscript writing. ML: data collection and manuscript review. W-XW: data analysis, manuscript writing and manuscript editing.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest regarding the contents of this article.
All procedures were conducted using protocols approved by the ethics committee of Wuhan University and were in accordance with the ARRIVE guidelines.
- 2.Petersen BT (2016) Post-endoscopic retrograde cholangiopancreatography pancreatitis during pregnancy: more questions than answers from administrative databases. Clin Gastroenterol Hepatol Off Clin Pract J Am Gastroenterol Assoc 14(1):115–117Google Scholar