Abstract
Purpose
In our work, furan, lycopene, and furan + lycopene treatments were applied to non-diabetic and diabetic female rats via gavage.
Methods
Ovarian tissue alterations with histopathology, immunohistochemistry, malondialdehyde levels, oxidative stress parameters such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and harmful effect on ovarian tissue DNA were evaluated in all groups for 28 days.
Results
Furan caused the changes histological, ovarian cell’s DNA structure, malondialdehyde levels, antioxidant enzymes activities as in a statistically significant manner in each group. Useful effect of lycopene was determined both in non-diabetic and diabetic treatment groups against furan according to the used experimental parameters. Although some histopathological alterations were seen in diabetic and non-diabetic/diabetic plus furan-treated group’s ovarians, lycopene restored these variations near to normal levels in furan + lycopene treated groups for in 28 days. Additionally, the results of our immunohistochemical analysis and alterations of the oxidative stress parameters results also supported these findings.
Conclusions
Our result confirms that lycopene has protective effect and significantly altered diabetes and furan-induced toxicity in the rat ovarian tissue.
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DP: manuscript writing, SU: manuscript editing, and DP: manuscript editing.
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This work was supported by the Bozok University Scientific Research Projects Unit by the code 6601-FBE/16-38.
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All authors declare that there is no conflict of interest.
Ethical approval
Female Wistar–Albino rats (300–320 g) were administrated according to standard protocol for use and care of laboratory animals. Çukurova University Animal Experiments Local Ethics Committee approved our treatment procedure (11/1).
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Uçar, S., Pandir, D. Furan induced ovarian damage in non-diabetic and diabetic rats and cellular protective role of lycopene. Arch Gynecol Obstet 296, 1027–1037 (2017). https://doi.org/10.1007/s00404-017-4521-7
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DOI: https://doi.org/10.1007/s00404-017-4521-7