Abstract
Purpose
Endometriosis has an incidence reaching up to 50% in infertile women. Cytokine-mediated immune responses seem to play an important role in endometriosis pathogenesis, but still the etiology and pathophysiology remain unclear. In the current study we tried to investigate whether there is a relationship between IL-10 genetic polymorphism, serum levels of IL-10 and the presence of advanced endometriosis.
Methods
The presence of IL-10 592C/A, 819T/C, 1082G/A promoter polymorphisms and IL-10 serum levels were investigated in advanced endometriosis patients compared to healthy controls. Genomic DNA was extracted from peripheral blood leukocytes and further analyzed by PCR.
Results
IL-10 serum levels were higher in endometriosis group compared to controls (1.48, 0.68, p < 0.001). We have observed an association between IL-10 592C/C and 819C/C genotypes, presence of C alleles and an increased risk of endometriosis. No difference was observed in IL-10 serum levels corresponding to different alleles or genotypes.
Conclusion
Our results suggest that IL-10 592A/C and 819T/C promoter polymorphisms confer susceptibility to advanced endometriosis. No associations were found between the IL-10 1082A/G polymorphism and susceptibility to advanced endometriosis.
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Acknowledgements
This paper was published under the project funded by “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania, internal Grant No. 4945/20/08.03.2016.
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This study was funded by “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania, internal Grant No. 4945/20/08.03.2016.
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A.M. Malutan declares that he has no conflict of interest. C. Drugan declares that she has no conflict of interest. K. Walch declares that she has no conflict of interest. T. Drugan declares that he has no conflict of interest. R. Ciortea declares that he has no conflict of interest. D. Mihu declares that he has no conflict of interest.
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All procedures performed in this study involving were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Informed consent was obtained from all individual participants included in the study.
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Malutan, A.M., Drugan, C., Walch, K. et al. The association between interleukin-10 (IL-10) −592C/A, −819T/C, −1082G/A promoter polymorphisms and endometriosis. Arch Gynecol Obstet 295, 503–510 (2017). https://doi.org/10.1007/s00404-016-4269-5
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DOI: https://doi.org/10.1007/s00404-016-4269-5