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The correlation of cumulus mucification patterns with oocyte maturation rate in vitro in FSH + LH-primed IVM cycles: a prospective study

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

This prospective study was conducted to compare the oocyte maturation rate in vitro among four types of cumulus cell (CC) morphology, in dual gonadotropin-primed in vitro maturation (IVM) oocyte cycles.

Methods

Two-hundred and thirty cumulus-oocyte complexes (COCs) were retrieved from FSH + hCG-primed in vitro maturation cycles of 20 patients diagnosed with polycystic ovarian syndrome. The COCs that contained immature oocytes were classified into four groups according to their cumulus mucification patterns (dispersed, clumped, compacted or sparse) and their oocyte maturation rates were compared. Chi square test and Fisher’s exact test were used as appropriate.

Results

Oocytes enclosed by dispersed and clumped CCs exhibited higher maturation rates than those with compacted and sparse patterns (P < 0.001).

Conclusion

In dual gonadotropin-primed IVM cycles, oocyte maturation rates are highest in those showing dispersed and clumped CC patterns.

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Acknowledgments

The authors would like to thank Ketsara Sitthirit and all the laboratory staff for their kind assistance. This research was funded by the Faculty of Medicine, Siriraj Hospital, Mahidol University.

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Authors and Affiliations

  1. Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand

    Isarin Thanaboonyawat, Orawan Makemaharn, Somsin Petyim, Pitak Laokirkkiat & Roungsin Choavaratana

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  1. Isarin Thanaboonyawat
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  2. Orawan Makemaharn
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Correspondence to Isarin Thanaboonyawat.

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Thanaboonyawat, I., Makemaharn, O., Petyim, S. et al. The correlation of cumulus mucification patterns with oocyte maturation rate in vitro in FSH + LH-primed IVM cycles: a prospective study. Arch Gynecol Obstet 293, 681–686 (2016). https://doi.org/10.1007/s00404-015-3935-3

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  • DOI: https://doi.org/10.1007/s00404-015-3935-3

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