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Simultaneous detection of human papillomavirus integration and c-MYC gene amplification in cervical lesions: an emerging marker for the risk to progression

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

The persistence of high-risk oncogenic human papillomavirus (HR-HPV) infection and its integration into the host genome are key steps in the induction of malignant alterations. c-MYC chromosome region is a frequent localization for HPV insertion that has been observed in chromosome band 8q24 by fluorescence in situ hybridization (FISH). We report the HPV viral integration and amplification patterns of the c-MYC gene in cytological smears with FISH as a potential biomarker for the progression of squamous intraepithelial lesions (SIL).

Methods

HPV detection and genotyping by polymerase chain reaction (PCR) and FISH analysis by “Vysis Cervical FISH Probe” kit (ABBOTT Molecular Inc.) were performed in 37 cervical samples including 8 NILM, 7 ASC-US, 7 LSIL, 3 ASC-H, 7 HSIL and 5 SCC.

Results

The results show concordance between FISH and PCR techniques for HPV detection. The majority of the samples contained HR-HPV, the majority being -16 and -18 genotypes. HPV integration as determined by FISH was most frequent in high-risk lesions. The c-MYC gene amplification was found only in HPV-positive samples and was detected primarily in high-risk lesions and in cells with an integrated form of HPV.

Conclusions

HPV integration and c-MYC gene amplification detected by FISH could be an important biomarker for use in clinical practice to determine SIL with a risk of progression.

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Acknowledgments

The author acknowledgements go to Abbott Company for providing the kits for realization of this research and Ph.D. Marcelo Gialluisi Bonini at College of Medicine, Departments of Medicine, Pharmacology and Pathology, University of Illinois at Chicago, for invaluable partnership and collaborations. This work was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES), Brazilian Government (PVE A109/2013 and AUX/PE-PRODOC 2571/2010).

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Correspondence to Vânia Ramos Sela da Silva.

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Gimenes, F., Souza, R.P., de Abreu, A.L.P. et al. Simultaneous detection of human papillomavirus integration and c-MYC gene amplification in cervical lesions: an emerging marker for the risk to progression. Arch Gynecol Obstet 293, 857–863 (2016). https://doi.org/10.1007/s00404-015-3870-3

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