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Measurement of oxidative stress in the follicular fluid of infertility patients with an endometrioma

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Follicular fluid (FF) might reflect the environment during follicle and oocyte growth, and an evaluation of oxidative stress in the FF might be useful in predicting oocyte quality. In order to measure the oxidative stress (OS) in the FF from a single follicle of patients with endometrioma (EM), we evaluated whether an EM might affect the environment of follicular growth.

Methods

Between December 2011 and July 2013, 26 patients with a unilateral EM (EM group) and 29 without EM (control group) were enrolled in this study. The FF was obtained during the first puncture of follicular aspiration, and was stored at −30 °C until it was assayed. A Free Radical Elective Evaluator (WISMERLL, USA) was used to perform d-ROM and BAP tests to measure oxidative stress (U.CARR) and antioxidant power (μmol/L).

Results

The d-ROM values in the EMC and control groups were 328.7 ± 97.8 and 414.9 ± 84.2, respectively, and the BAP values for the two groups were 2474.3 ± 432.0 and 2552.8 ± 435.58, respectively. These values were similar between the two groups (mean ± SD). The number of patients with a modified BAP/d-ROM ratio of <1.0 in the EM group was similar to that for the control group at 16 and 15, respectively (61.5 and 51.7 %).

Conclusions

The oxidative stress and antioxidant potential in the FF of the patients with unilateral EM showed values similar to those without an EM. Therefore, we concluded that EMs do not affect the environment for follicle growth during ART treatment.

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Authors and Affiliations

  1. Division of Reproductive Medicine, Sugiyama Clinic, 1-53-1, Ohara, Setagaya, Tokyo, 156-0041, Japan

    Koji Nakagawa & Rikikazu Sugiyama

  2. Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo, Japan

    Michi Hisano & Koushi Yamaguchi

Authors
  1. Koji Nakagawa
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  2. Michi Hisano
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  3. Rikikazu Sugiyama
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  4. Koushi Yamaguchi
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Correspondence to Koji Nakagawa.

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The authors have received no funding for this study, and they have no financial interest in any companies.

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Nakagawa, K., Hisano, M., Sugiyama, R. et al. Measurement of oxidative stress in the follicular fluid of infertility patients with an endometrioma. Arch Gynecol Obstet 293, 197–202 (2016). https://doi.org/10.1007/s00404-015-3834-7

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  • DOI: https://doi.org/10.1007/s00404-015-3834-7

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