The aim of the current study is to determine, correlate and compare the plasma lipoprotein-associated phospholipase A2 (Lp-PLA2), vitronectin (Vn), Plasminogen activator inhibitor-1 (PAI-1) activity and tissue plasminogen activator (t-PA) levels in early-onset preeclampsia, late-onset preeclampsia and in control pregnant women.
A total of 79 individuals, 30 early-onsets, and 22 late-onset preeclamptic and 27 control pregnant women were included into the scope of this study. Enzyme-linked immunosorbent assay procedure was used to determine the serum Lp-PLA2 and plasma Vn, t-PA antigen and PAI-1 activity levels. Serum C-reactive protein (CRP) levels were measured immunoturbidimetrically in routine clinical chemistry analyser.
In patients with preeclampsia, Lp-PLA2, PAI-1, t-PA, CRP and blood pressures levels were increased (p = 0.000) and correlated with each other. Vn levels were decreased (p = 0.016) but not correlated with other parameters in preeclamptic patients.
We are of the opinion that increased Lp-PLA2 levels may partially contribute to endothelial dysfunction by the progression of inflammation. In addition, increased complex formation with Vn is likely to bring about the increase of PAI-1 activity in patients with preeclampsia. Moreover, increased t-PA and decreased Vn levels may also be the consequences of compensatory mechanisms against disease progression.
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ACOG Committee on Obstetric Practice (2002) ACOG practice bulletin. Diagnosis and management of preeclampsi and eclampsia. Number 33, January 2002. American College of Obsteticians and Gynecologists. Int J Gynaecol Obstet 77(1):67–75
Weiss G, Goldsmith LT, Taylor RN, Bellet D, Taylor HS (2009) Inflammation in reproductive disorders. Reprod Sci 16(2):216–229
Fan P, Liu XH, He GL, Zhang S, Zhang JX, Bai H (2012) Maternal and fetal plasma platelet-activating factor acetylhydrolase activity and distribution in pre-eclampsia. Pediatr Res 72(4):426–431
Ramma W, Ahmed A (2011) Is inflammation the cause of pre-eclampsia? Biochem Soc Trans 39(6):1619–1627
Dusse LM, Rios DR, Pinheiro MB, Cooper AJ, Lwaleed BA (2011) Pre-eclampsia: relationship between coagulation, fibrinolysis and inflammation. Clin Chim Acta 412(1–2):17–21
Boij R, Svensson J, Nilsson-Ekdahl K, Sandholm K, Lindahl TL, Palonek E, Garle M, Berg G, Ernerudh J, Jenmalm M, Matthiesen L (2012) Biomarkers of coagulation, inflammation, and angiogenesis are independently associated with preeclampsia. Am J Reprod Immunol 68(3):258–270
Abbate R, Cioni G, Ricci I, Miranda M, Gori AM (2012) Thrombosis and acute coronary syndrome. Thromb Res 129(3):235–240
Yang EH, McConnell JP, Lennon RJ, Barsness GW, Pumper G, Hartman SJ, Rihal CS, Lerman LO, Lerman A (2006) Lipoprotein-associated phospholipase A2 is an independent marker for coronary endothelial dysfunction in humans. Arterioscler Thromb Vasc Biol 26(1):106–111
Lavi S, McConnell JP, Rihal CS, Prasad A, Mathew V, Lerman LO, Lerman A (2007) Local production of lipoprotein-associated phospholipase A2 and lysophosphatidylcholine in the coronary circulation: association with early coronary atherosclerosis and endothelial dysfunction in humans. Circulation 115(21):2715–2721
Garg PK, McClelland RL, Jenny NS, Criqui M, Liu K, Polak JF, Jorgensen NW, Cushman M (2011) Association of lipoprotein-associated phospholipase A (2) and endothelial function in the Multi-Ethnic Study of Atherosclerosis (MESA). Vasc Med 16(4):247–252
Aso Y (2007) Plasminogen activator inhibitor (PAI)-1 in vascular inflammation and thrombosis. Front Biosci 12:2957–2966
Wikström AK, Nash P, Eriksson UJ, Olovsson MH (2009) Evidence of increased oxidative stress and a change in the plasminogen activator inhibitor (PAI)-1 to PAI-2 ratio in early-onset but not late-onset preeclampsia. Am J Obstet Gynecol 201(6):597.e1–597.e8
Ekmekçi OB, Ekmekçi H (2006) Vitronectin in atherosclerotic disease. Clin Chim Acta 368(1–2):77–83
ACOG Committee on Obstetric Practice (2002) ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002. Obstet Gynecol 99(1):159–167
Greer IA, Dawes J, Johnston TA, Calder AA (1991) Neutrophil activation is confined to the maternal circulation in pregnancy-induced hypertension. Obstet Gynecol 78(1):28–32
Lynch AM, Murphy JR, Gibbs RS, Levine RJ, Giclas PC, Salmon JE, Holers VM (2010) The interrelationship of complement-activation fragments and angiogenesis-related factors in early pregnancy and their association with pre-eclampsia. BJOG 117(4):456–462
Szarka A, Rigó J Jr, Lázár L, Beko G, Molvarec A (2010) Circulating cytokines, chemokines and adhesion molecules in normal pregnancy and preeclampsia determined by multiplex suspension array. BMC Immunol 11:59
Renckens R, Roelofs JJ, de Waard V, Florquin S, Lijnen HR, Carmeliet P, van der Poll T (2005) The role of plasminogen activator inhibitor type 1 in the inflammatory response to local tissue injury. J Thromb Haemost 3(5):1018–1025
Chappell LC, Seed PT, Briley A, Kelly FJ, Hunt BJ, Charnock-Jones DS, Mallet AI, Poston L (2002) A longitudinal study of biochemical variables in women at risk of preeclampsia. Am J Obstet Gynecol 187(1):127–136
Tabak O, Gelisgen R, Uzun H, Kalender B, Balci H, Curgunlu A, Simsek G, Karter Y (2009) Hypertension and hemostatic/fibrinolytic balance disorders. Clin Invest Med 32(6):E285–E292
Tanjung MT, Siddik HD, Hariman H, Koh SC (2005) Coagulation and fibrinolysis in preeclampsia and neonates. Clin Appl Thromb Hemost 11(4):467–473
Shen J, Ma X, Yi F, Rasul A, Cui M, Ma T (2013) Increased expression levels of vitronectin in the maternal fetal interface of placenta in early-onset severe preeclampsia. Mol Med Rep 7(1):53–58
Asch E, Podack E (1990) Vitronectin binds to activated human platelets and plays a role in platelet aggregation. J Clin Invest 85(5):1372–1378
Mohri H, Ohkubo T (1991) How vitronectin binds to activated glycoprotein IIb-IIIa complex and its function in platelet aggregation. Am J Clin Pathol 96(5):605–609
Reheman A, Gross P, Yang H, Chen P, Allen D, Leytin V, Freedman J, Ni H (2005) Vitronectin stabilizes thrombi and vessel occlusion but plays a dual role in platelet aggregation. J Thromb Haemost 3(5):875–883
This study was supported by Istanbul University, Scientific Research Projects Unit (Project Number: BYP-20082).
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Balcı Ekmekçi, Ö., Ekmekçi, H., Güngör, Z. et al. Evaluation of Lp-PLA2 mass, vitronectin and PAI-1 activity levels in patients with preeclampsia. Arch Gynecol Obstet 292, 53–58 (2015). https://doi.org/10.1007/s00404-014-3601-1