Abstract
Purpose
The objective of this study is to quantify and evaluate the expression of several important proteins in TGF-β1/Smad pathway in the anterior vaginal wall in postpartum rats with stress urinary incontinence (SUI).
Methods
Forty 8-week-old Sprague–Dawley (SD) female rats were randomized into three groups: blank group (n = 10), control group (n = 10) and SUI group (n = 20). Rats in blank group were non-pregnant, while rats in the control and SUI groups underwent normal parturition and normal parturition plus immediate postpartum vaginal balloon dilation, respectively. 1 week after dilation, a sneezing experiment and pad test were performed and the anterior vaginal wall was collected. The histological changes of the anterior vaginal wall were assessed by hematoxylin–eosin (HE) staining, and the expression of TβR-2, Smad3 and Smad7 in the anterior vaginal wall was detected by immunohistochemical staining and Western blotting.
Results
HE staining showed that collagen was more fragmented, sparse and disorganized in the SUI group compared with the control and blank groups. Compared with the blank group, the expression of TβR-2 and Smad7 protein was significantly increased in the vaginal anterior wall in the control and SUI groups (P < 0.05), while their levels in the SUI group were significantly higher than those in the control group (P < 0.05). Expression of Smad3 protein in the anterior vaginal wall of SUI rats was significantly decreased compared with the blank and control groups (P < 0.05).
Conclusion
Dysregulation of the TGF-β1/Smad signaling pathway may involve in the pathogenesis of SUI.
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Abbreviations
- SUI:
-
Stress urinary incontinence
- IHC:
-
Immunohistochemistry
- ECM:
-
Extracellular matrix
- TGF-β1:
-
Transforming growth factor-β1
- TβR-2:
-
Transforming growth factor receptor II
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Acknowledgments
This study was supported by a Grant from the Science Technology and Information Bureau of Guangzhou (Grant No. 201300000137) and a fund for Excellent Talents in the Third Affiliated Hospital of Guangzhou Medical University.
Conflict of interest
We declare that we have no conflict of interest. We state that we have had full control of all primary data and we agree to allow the Journal to review our data if requested.
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Wang, H., Liu, J., Zeng, J. et al. Expression of TβR-2, Smad3 and Smad7 in the vaginal anterior wall of postpartum rats with stress urinary incontinence. Arch Gynecol Obstet 291, 869–876 (2015). https://doi.org/10.1007/s00404-014-3495-y
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DOI: https://doi.org/10.1007/s00404-014-3495-y