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The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3′UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population

  • Maternal-Fetal Medicine
  • Published:
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Abstract

Purpose

To investigate the main effect of polymorphisms in genes involved in endothelial pathophysiological mechanisms, LOX-1 K167N and 3′UTR188CT single nucleotide polymorphisms (SNPs) in relation to preeclampsia (PE) risk and possible interactions between the gene polymorphisms and plasma oxLDL and soluble LOX-1 (sLOX-1) levels on PE in Turkish population.

Methods

LOX-1 K167N and 3′UTR188CT polymorphisms were studied in 113 pregnant women with preeclampsia and 96 healthy pregnant women by the PCR–RFLP techniques. sLOX-1 and oxLDL levels were determined by enzyme-linked immunosorbent assay (ELISA) in all study subjects.

Results

Patients having LOX-1 3′UTR188CT (OR 3.55, 95 % CI 1.89–6.67, P = 0.001) or 3′UTR188CC (OR 3.04, 95 % CI 1.25–7.38, P = 0.012) genotype had a significantly higher risk of PE than those with 3′UTR188TT genotype. Also, patients having K167N KK (OR 2.73, 95 % CI 1.33–5.61, P = 0.005) genotype had a significantly higher risk of PE than those with K167N NN genotype. LOX-1 3′UTR188TT and LOX-1 K167N NN genotype carriers were associated with significantly increased serum sLOX-1 level (P = 0.001). We further investigated the potential combined effect of these polymorphic variants on risk of PE development. According to the combined genotype analysis of LOX-1 3′UTR188TT and K167N NN polymorphisms, sLOX-1 and oxLDL levels also showed significant differences between PE patients and controls with or without combined TT/NN genotype carriers.

Conclusions

Our findings indicate that higher plasma sLOX-1 and oxLDL concentrations, and the LOX-1 3′UTR188C>T and LOX-1 K167N gene polymorphisms were significantly associated with risk of developing preeclampsia. Plasma sLOX-1 may be a potential therapeutic target in the treatment of preeclampsia.

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Acknowledgments

This work was supported by the Research Fund of the University of Istanbul, project number BYP-21176 and Istanbul University Research Fund (BAP).

Conflict of interest

The authors declare that they have no conflicts of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey

    Abdullah Tuten, Mahmut Oncul & Abdullah Serdar Acıkgoz

  2. Department of Biophysics, Medical Faculty, Sakarya University, Korucuk Campus, 54290, Korucuk Adapazari, Sakarya, Turkey

    Birsen Aydemir

  3. Department of Biophysics, Medical Faculty, Namık Kemal University, 59100, Tekirdag, Turkey

    Ali Riza Kiziler

  4. Kırklareli University, School of Health, 39020, Kırklareli, Turkey

    Gulcan Guntas Korkmaz

  5. Department of Biochemistry, Yildiz Technical University, 34220, Istanbul, Turkey

    Volkan Sozer

  6. Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey

    Hafize Uzun

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  1. Abdullah Tuten
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Correspondence to Birsen Aydemir.

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Tuten, A., Aydemir, B., Oncul, M. et al. The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3′UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population. Arch Gynecol Obstet 291, 563–571 (2015). https://doi.org/10.1007/s00404-014-3457-4

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  • DOI: https://doi.org/10.1007/s00404-014-3457-4

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