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MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Introduction

Focal adhesion kinase (FAK) is a critical mediator of extracellular matrix signaling, cell survival, proliferation and motility. Twist homolog 1 (TWIST1) is a transcription factor and serves as a powerful oncogene. Increased FAK and TWIST1 expression are observed in a variety of solid human tumors and correlate with metastasis and poor survival.

Materials and methods

Here, we identify miR-543 as a direct regulator of FAK and TWIST1 expression in endometrial cancer.

Results

Forced expression of miR-543 in endometrial cancer cell lines decreases both endogenous FAK and TWIST1 mRNA and protein levels. Forced expression of miR-543 in aggressive endometrial cancer cell lines also impairs tumor cell monolayer proliferation, anchorage-independent growth, migration and invasion. Endogenous miR-543 expression is decreased in malignant versus normal endometrium tissue and the levels of miR-543 inversely correlate with mRNA levels of FAK and TWIST1.

Conclusions

miR-543 expression is decreased in endometrial cancer and serves as a tumor suppressor by targeting FAK and TWIST1 expression.

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Abbreviations

FAK:

Focal adhesion kinase

TWIST1:

Twist homolog 1

miRNA:

MicroRNA

BrdU:

5-Bromo-2-deoxyuridine

EMT:

Epithelial mesenchymal transition

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Acknowledgments

This work was supported by the Natural Scientific Research Fund of China (No. 81100916).

Conflict of interest

We declare that we have no conflict of interests.

Author information

Authors and Affiliations

  1. Department of Gynecology and Obstetrics, The Military General Hospital of Beijing PLA, Beijing, 100700, China

    Li Bing & Shang Li-Xin

  2. Department of Anesthesiology, The Military General Hospital of Beijing PLA, Beijing, 100700, China

    Chen Hong

  3. Department of Gynecology, Beijing Maternity Hospital, No. 251, Yaojiayuan Road, Chaoyang District, Beijing, 100026, China

    Gao Wei

Authors
  1. Li Bing
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  2. Chen Hong
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  3. Shang Li-Xin
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  4. Gao Wei
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Corresponding author

Correspondence to Gao Wei.

Additional information

Dr. C. Hong and Dr. L. Bing contributed equally to this study.

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Bing, L., Hong, C., Li-Xin, S. et al. MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression. Arch Gynecol Obstet 290, 533–541 (2014). https://doi.org/10.1007/s00404-014-3219-3

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  • DOI: https://doi.org/10.1007/s00404-014-3219-3

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