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Sham-controlled implantation after preimplantation genetic screening by polar body biopsy and FISH

  • Maternal-Fetal Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Preimplantation genetic screening wants to improve artificial reproductive technologies, primarily by raising the rates of pregnancy, implantation and birth. We investigated if embryos derived from oocytes detected euploid for five chromosomes implant better than those which were biopsied but where the genetic detection failed. They were nevertheless transferred, thus serving as a sham control.

Method

From 2004 to 2008 we performed 104 cycles of PGS with laser biopsy of the first polar body and FISH with five chromosomes. It was offered to all patients with eight or more oocytes, free of charge. The average female age was 36 years. If no euploid oocytes were available, not detected oocytes were transferred.

Result

In 104 cycles 99 embryo transfers (95 %) were performed, resulting in 28 pregnancies (27 %), 20 births (71 %) and 8 miscarriages (29 %). The implantation rate in the euploid group was 19 vs. 13 % in the not detected group (n.s.). This trend was the same independent of age and embryo morphology.

Conclusion

The pregnancy rate does not differ significantly from the national average. The trend in better implantation rates of euploid oocytes justifies a continuation of studies in this matter.

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Acknowledgments

We thank Santiago Munne and Dr. Chen Wen for helping us to establish our PGS program, Dr. Melanie Glander for starting the PGS laboratory part, Prof. Dr. Reiner Siebert and Dr. Almuth Caliebe, University Institute for Human Genetics, for support of the study by genetic counselling and Ms Kerstin Koch for her diligent work in the lab and her meticulous acquisition of data.

Conflict of interest

All authors declare no conflict of interest.

Study registration

The study was registered and approved by the Ethics Commission of the Medical Faculty of the Christian-Albrechts-University Kiel.

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Authors and Affiliations

  1. Women’s Hospital, Christian-Albrechts-University, Kiel, Germany

    Andreas G. Schmutzler, Bengi Acar-Perk, Jörg Weimer, Ali Salmassi, Knud Sievers, Monica Tobler, Liselotte Mettler, Walter Jonat & Norbert Arnold

  2. Gyn-Medicum Fertility Center, Göttingen, Germany

    Monica Tobler

  3. Universitätsfrauenklinik, Arnold-Heller Str. 3, Haus 24, 24105, Kiel, Germany

    Andreas G. Schmutzler

Authors
  1. Andreas G. Schmutzler
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  2. Bengi Acar-Perk
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  3. Jörg Weimer
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  4. Ali Salmassi
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  5. Knud Sievers
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  6. Monica Tobler
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  7. Liselotte Mettler
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  8. Walter Jonat
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  9. Norbert Arnold
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Corresponding author

Correspondence to Andreas G. Schmutzler.

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Schmutzler, A.G., Acar-Perk, B., Weimer, J. et al. Sham-controlled implantation after preimplantation genetic screening by polar body biopsy and FISH. Arch Gynecol Obstet 289, 439–444 (2014). https://doi.org/10.1007/s00404-013-2945-2

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  • DOI: https://doi.org/10.1007/s00404-013-2945-2

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