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Establishment of Fischer 344 rat model of ovarian cancer with lymphatic metastasis

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To establish a model of ovarian cancer with highly lymphatic metastasis in immunocompetent rats.

Methods

Thirty-two female Fischer 344 rats were divided randomly and equally into two groups: footpad group and intraperitoneal (i.p.) group. At 8 weeks after injection with NuTu-19 ovarian cancer cells, lymphatic metastasis were analyzed by pathohistology; body weight was monitored per week, Survival curves were determined by Kaplan–Meier analysis.

Results

Footpad injection could efficiently generate the lymphatic metastasis; specifically, the incidences of metastasis in the ipsilateral popliteal, inguinal and para-iliac lymph nodes were 100 % (8/8), 75 % (6/8), and 37.5 % (3/8), respectively. The mean volume and weight of the ipsilateral popliteal lymph nodes were 0.405 ± 0.096 cm3 and 0.418 ± 0.118 g in footpad group. However, no lymphatic metastasis lesions were found in i.p. group. Moreover, Kaplan–Meier analysis showed that the average survival time of the footpad group was significantly longer than that of the i.p. group (18.429 ± 1.112 vs. 10.286 ± 0.505 weeks).

Conclusions

Our experiments suggest that footpad injection is a very efficient method to generate ovarian cancer with lymphatic metastasis in an immune-competent animal, and we believe that this model will be very helpful for shedding light on the mechanism of lymphogenous metastasis and developing novel therapeutic targets for ovarian cancer patients.

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Acknowledgments

We would like to acknowledge the guidance of the Department of Laboratory Animal Science in Fudan University, and especially thank Mr. Weiwei Qiao and Mr. Xianzhe Zhao for their invaluable support and assistance. We would also like to thank the members of our research team who provided useful comments throughout the project.

Conflict of interest

No conflict of interest to declare.

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Authors and Affiliations

  1. Obstetrics and Gynecology Hospital, Department of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, No.419 Fangxie Road, Shanghai, 200011, China

    Lingling Fan, Yingtao Liu, Xiaoyan Zhang, Yu Kang & Congjian Xu

  2. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China

    Lingling Fan, Yingtao Liu, Xiaoyan Zhang, Yu Kang & Congjian Xu

Authors
  1. Lingling Fan
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  2. Yingtao Liu
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  3. Xiaoyan Zhang
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  4. Yu Kang
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  5. Congjian Xu
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Corresponding author

Correspondence to Congjian Xu.

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Fan, L., Liu, Y., Zhang, X. et al. Establishment of Fischer 344 rat model of ovarian cancer with lymphatic metastasis. Arch Gynecol Obstet 289, 149–154 (2014). https://doi.org/10.1007/s00404-013-2937-2

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  • DOI: https://doi.org/10.1007/s00404-013-2937-2

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