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Detection of Human Papillomavirus in biopsies of patients with cervical cancer, and its association with prognosis

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Objective

To assess the detection of Human Papillomavirus (HPV) in cervical biopsies, and its association with clinical response and survival of patients with cervical cancer.

Method

The presence of HPV was determined by polymerase chain reaction (nested PCR) of material from 183 paraffin blocks containing biopsies of patients with cervical cancer. Directives of the International Federation of Gynecology and Obstetrics (FIGO) were used for the clinical staging of all cases. Survival analysis was conducted with the Kaplan–Meier method and differences between the curves were calculated by the log-rank test.

Results

Amplification was positive in 78 cases, negative in 96 cases and indeterminate in 9. The detection of HPV had no statistically significant effect on the survival of patients.

Conclusions

From amongst the variables analyzed, only HPV positivity and clinical staging influenced a complete response after completing treatment. However, clinical staging was the only factor associated with progression of the disease, with patients in the early stages presenting better overall survival rates (p < 0.001).

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Authors and Affiliations

  1. Oncology Unit, Hospital Universitário Oswaldo Cruz, Recife, PE, Brazil

    Carla Limeira Barreto

  2. Keizo Asami Immunopathology Laboratory, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Danyelly Bruneska Gondim Martins & José Luiz de Lima Filho

  3. Post-graduation Program in Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Vera Magalhães

  4. Rua Arnóbio Marques, 310, Santo Amaro, Recife, PE, CEP 52011-180, Brazil

    Carla Limeira Barreto

Authors
  1. Carla Limeira Barreto
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  2. Danyelly Bruneska Gondim Martins
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  3. José Luiz de Lima Filho
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  4. Vera Magalhães
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Correspondence to Carla Limeira Barreto.

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Barreto, C.L., Martins, D.B.G., de Lima Filho, J.L. et al. Detection of Human Papillomavirus in biopsies of patients with cervical cancer, and its association with prognosis. Arch Gynecol Obstet 288, 643–648 (2013). https://doi.org/10.1007/s00404-013-2803-2

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  • DOI: https://doi.org/10.1007/s00404-013-2803-2

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