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The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth

  • Maternal-Fetal Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Periodontal disease is considered to be a risk factor for threatened preterm labor (TPL) and preterm birth (PB), but pathogenic mechanisms have not yet been elucidated. We hypothesized that infection with periodontopathic bacteria may enhance thrombosis through molecular mimicry with TLRVYK peptides on beta-2 glycoprotein I, a target molecule in anti-phospholipid syndrome. This study aimed to examine the effects of periodontitis on TPL and PB.

Methods

Ninety-five pregnant women (47 TPL and 48 healthy subjects) participated. Periodontal clinical parameters and periodontopathic bacteria were examined. Molecular mimicry between TLRVYK peptides and homologous peptides on the periodontopathic bacteria was examined by enzyme-linked immunosorbent assay (ELISA) using rabbit polyclonal antibodies specific for the respective peptides (SIRVYK on Aggregatibacter actinomycetemcomitans, TLRIYT on Porphyromonus gingivalis, and TLALYK on Treponema denticola). Serum high-sensitivity C-reactive protein, anti-TLRVYK and anti-SIRVYK IgG antibodies were measured using ELISA.

Results

Among the rabbit antibodies specific for the bacterial homologous peptides, only anti-SIRVYK IgG antibody reacted with TLRVYK peptides. Multivariable analysis showed that anti-SIRVYK IgG antibody was significantly associated with diagnosis of TPL. Of 95 births, 14 (14.7 %) delivered preterm. The preterm birth rate was higher in the anti-SIRVYK IgG antibody >median group than in the ≤median group. Of the 47 TPL subjects 13 had PB, and ordinal logistic regression analysis revealed that past smoking, presence of P. gingivalis and anti-SIRVYK IgG antibody were significantly correlated with PB.

Conclusions

Infection with P. gingivalis and the antibody response to SIRVYK might be associated with TPL and PB.

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Acknowledgments

We are grateful to all the study participants. This study was supported by a Grant-in-Aid for Young Scientists (B) (21792111), and the 8020 Promotion Foundation. The authors declare that there is no conflict of interest associated with this manuscript.

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Authors and Affiliations

  1. Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Changchang Ye, Sayaka Katagiri, Pariksha Bharti, Hiroaki Kobayashi, Yasuo Takeuchi & Yuichi Izumi

  2. Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

    Changchang Ye, Pariksha Bharti & Yuichi Izumi

  3. Department of Pediatrics, Perinatal and Maternal Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

    Naoyuki Miyasaka & Yoshihito Momohara

  4. Maternal and Women’s Clinic, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan

    Masaki Sekiguchi & Satoko Takamine

  5. Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Sapporo, Japan

    Toshiyuki Nagasawa

Authors
  1. Changchang Ye
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  2. Sayaka Katagiri
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  3. Naoyuki Miyasaka
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Correspondence to Sayaka Katagiri.

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Ye, C., Katagiri, S., Miyasaka, N. et al. The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth. Arch Gynecol Obstet 288, 65–72 (2013). https://doi.org/10.1007/s00404-013-2741-z

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  • DOI: https://doi.org/10.1007/s00404-013-2741-z

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