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Evaluation of DAPK gene methylation and HPV and EBV infection in cervical cells from patients with normal cytology and colposcopy

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Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Objective

Evaluation of promoter methylation of the death-associated protein kinase (DAPK) gene and HPV and EBV infections in cervical cells from patients with normal cytology and colposcopy.

Study design

Twenty women, who had been patients at the Institute of Gynecology of the Federal University of Rio de Janeiro (UFRJ) for routine examinations and who showed normal cytology and colposcopy, were selected for this work. Cervical brushings were used for DNA extraction, and the analysis of methylation patterns of the DAPK gene was done through chemical modification with sodium bisulfite. Analysis of viral infection was done using polymerase chain reaction (PCR).

Results

Of the 20 patients studied, six (30%) presented methylation of the DAPK gene, five (25%) presented infection with EBV and three (15%) presented coinfection with HPV/EBV. Associating methylation with viral infection, we found methylated DAPK in one patient (16%) with EBV, in two patients (33%) with co-infection and in three patients (50%) with no viral infection.

Conclusions

In the present study, we verified, for the first time, the methylation pattern of the DAPK gene in cervical smears from patients with normal cytology and colposcopy. The results also showed the presence of viral infections in these patients. EBV infection, irrespective of whether associated with HPV or not, may contribute to cervical carcinogenesis as a cofactor. Methylation of the DAPK gene is associated with cell transformation, suggesting that DAPK methylation might be an important marker for the development of cervical epithelial neoplasias.

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Acknowledgments

Our thanks to Brenda Maiolino Bucco and Marcelo Soares da Mota e Silva for their assistance during development of the study, and to Siane Lopes Bittencourt Rosas for her professional review. This study was financed by the National Council for Scientific and Technological Development (CNPq) and by the Ary Frauzino Foundation for Cancer Research and Prevention.

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Authors and Affiliations

  1. Genetic Expression Control Laboratory, Carlos Chagas Filho Institute of Biophysics, UFRJ, Centro de Ciências da Saúde (CCS), Bloco G, Ilha do Fundão, Rio de Janeiro, RJ, CEP 21949-900, Brazil

    Fernanda Lattario & Maria da Gloria da Costa Carvalho

  2. Institute of Gynecology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Yara Lucia Furtado, Filomena Aste Silveira, Isabel Cristina do Val & Gutemberg Almeida

Authors
  1. Fernanda Lattario
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  2. Yara Lucia Furtado
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  3. Filomena Aste Silveira
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  4. Isabel Cristina do Val
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  5. Gutemberg Almeida
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  6. Maria da Gloria da Costa Carvalho
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Correspondence to Fernanda Lattario.

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Lattario, F., Furtado, Y.L., Silveira, F.A. et al. Evaluation of DAPK gene methylation and HPV and EBV infection in cervical cells from patients with normal cytology and colposcopy. Arch Gynecol Obstet 277, 505–509 (2008). https://doi.org/10.1007/s00404-007-0511-5

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  • DOI: https://doi.org/10.1007/s00404-007-0511-5

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