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BMP-2 regulates the expression of myosin Va via smad in melan-a melanocyte

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Abstract

Myosin Va (Myo Va) is one of three protein complexes involved in melanosome transport. In this study, we identified BMP-2 as an up-regulator of Myo Va expression using 2-methyl-naphtho[1,2,3-de]quinolin-8-one (MNQO). Our results showed that MNQO reduced the mRNA and protein expression of Myo Va and BMP-2 in melanocytes. Knockdown of BMP-2 by siRNA also affected Myo Va mRNA and protein expression, confirming that MNQO regulates Myo Va through BMP-2. Furthermore, phosphorylation of Smad1/5/8 by BMP2 treatment confirmed that the BMP-2/Smad signaling pathway regulates Myo Va expression in Melan-a melanocytes. Smad-binding elements were found in the Myo Va promoter and phosphorylated Smad1/5/8 bind directly to the Myo Va promoter to activate Myo Va transcription and BMP-2 enhances this binding. These findings provide insight into a new role for BMP-2 in Melan-a melanocytes and a mechanism of regulation of Myo Va expression that may be beneficial in the treatment of albinism or hyperpigmentation disorders.

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No datasets were generated or analysed during the current study.

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Acknowledgements

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HP23C0001).

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Authors and Affiliations

  1. Department of Genetics & Biotechnology and Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446-701, Gyeonggi-do, South Korea

    Ji Yun Park, Chan Song Jo, ChangHoe Ku & Jae Sung Hwang

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  1. Ji Yun Park
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  2. Chan Song Jo
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  3. ChangHoe Ku
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  4. Jae Sung Hwang
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Contributions

Conceptualization, JS.H and JY.P; methodology, JY.P and CS.J; formal analysis, JY.P; investigation, JY.P; resources, JY.P and CH. K; data curation, CS.J and JY.P; writing—original draft preparation, JY.P; writing—review and editing, CS.J; supervision, JS.H; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jae Sung Hwang.

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Park, J.Y., Jo, C.S., Ku, C. et al. BMP-2 regulates the expression of myosin Va via smad in melan-a melanocyte. Arch Dermatol Res 316, 225 (2024). https://doi.org/10.1007/s00403-024-02955-9

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  • DOI: https://doi.org/10.1007/s00403-024-02955-9

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