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Circular RNA hsa_Circ_0005795 mediates cell proliferation of cutaneous basal cell carcinoma via sponging miR-1231

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Abstract

Growing evidence has revealed that circular RNAs (circRNA) play critical roles in cancer progression. Here, we examined the function of a novel circRNA, Circ_0005795, in basal cell carcinoma (BCC) and explored the possible molecular mechanism. Nodular BCC and adjacent non-tumor tissues derived from 30 patients and 2 BCC cell lines were applied to analyze gene expression. Circ_0005795 loss- and gain-of-function were constructed to investigate BCC progression. Nuclear and cytoplasmic fractionation and luciferase assay were carried out to determine cellular localization and molecular interaction of Circ_0005795. Circ_0005795 expression was significantly elevated in BCC tissues and cells. Knockdown of Circ_0005795 dramatically reduced cell viability, colony formation, and anti-apoptotic protein levels, while increased caspase-3 activity. Circ_0005795 located in cytoplasm, which exerted its tumor-promoting effect through targeting and sponging miR-1231 in BCC cells. In summary, Circ_0005795 works as an oncogene in BCC, which might be used as a promising biomarker and a potential therapeutic target for BCC diagnosis and treatment.

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Authors and Affiliations

  1. Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Yong’an Road, Beijing, 100050, China

    Yating Li & Linfeng Li

  2. Department of Dermatology, Qingdao Municipal Hospital, Jiaozhou Road, Qingdao, 266011, Shandong, China

    Yating Li & Yang Li

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  1. Yating Li
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Correspondence to Linfeng Li.

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All the patients were obtained informed consent following a protocol approved by the ethics board of Qingdao Municipal Hospital, and this study was conducted in accordance with the ethical standards formulated in the Declaration of Helsinki.

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Li, Y., Li, Y. & Li, L. Circular RNA hsa_Circ_0005795 mediates cell proliferation of cutaneous basal cell carcinoma via sponging miR-1231. Arch Dermatol Res 313, 773–782 (2021). https://doi.org/10.1007/s00403-020-02174-y

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  • DOI: https://doi.org/10.1007/s00403-020-02174-y

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