Archives of Dermatological Research

, Volume 311, Issue 6, pp 443–452 | Cite as

Development and evaluation of a vegetable oil blend formulation for cutaneous wound healing

  • Marcio Guidoni
  • Mariana Moreira Figueira
  • Gabrielly Pereira Ribeiro
  • Dominik Lenz
  • Pamela Aparecida Grizotto
  • Thiago de Melo Costa Pereira
  • Rodrigo Scherer
  • Stanislau BoguszJr.
  • Marcio FronzaEmail author
Original Paper


This work aimed to evaluate the in vivo capacity of a vegetable oil blend formulation (VOB) developed to accelerate cutaneous wound closure. Total thickness wounds were punctured on the skin on the back side of each animal and topically treated with the VOB formulation, Dersani® ointment or the vehicle control. After 2, 7, 14, 21 days post-wounding, five animals from each group were euthanized, and the rates of wound closure and re-epithelialization were evaluated. The wounds were harvested for histological and biochemical analysis. VOB resulted in faster and greater re-epithelialization in the in vivo excisional wounds, exhibiting significant wound area reduction of 8.9, 8.0, 35.1, 45.2 and 47.0% after 2, 5, 10, 14 and 21 days post-wounding, respectively, when compared with the vehicle control. Histological and biochemical analyses showed that the VOB-treated wounds exhibited a significant increase of granular tissue and controlled inflammatory response by modulation of the release of pro-inflammatory cytokines TNF-α, IL-6 and IL-1. Moreover, VOB-treated wounds showed a significant and concrete increase in the deposition and organisation of collagen fibres in the wound site and improved the quality of the scar tissue. Altogether, these data revealed that VOB accelerates wound healing processes and might be beneficial for treating wound disorders.


Fatty acids Vegetable oil Wound healing Cytokines Collagen Inflammation 



The authors wish to thank the Fundação de Amparo à Pesquisa do Espirito Santo (FAPES), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This work follows ethical standards and all experiments involving the use of animals were conducted in agreement with the Brazilian Animal Care Committee and were approved by the Committee of Ethics, Bioethics and Animal Welfare of the Universidade Vila Velha (UVV) (CEUA-UVV protocol 381/2016).

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Marcio Guidoni
    • 1
  • Mariana Moreira Figueira
    • 1
  • Gabrielly Pereira Ribeiro
    • 1
  • Dominik Lenz
    • 1
  • Pamela Aparecida Grizotto
    • 2
  • Thiago de Melo Costa Pereira
    • 1
  • Rodrigo Scherer
    • 1
  • Stanislau BoguszJr.
    • 2
  • Marcio Fronza
    • 1
    Email author
  1. 1.Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos NaturaisUniversidade Vila Velha-UVVEspírito SantoBrazil
  2. 2.Instituto de Química de São Carlos-IQSC, Universidade de São Paulo-USPSão CarlosBrazil

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