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Heme oxygenase and iron status in exosomes of psoriasis patients

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Abstract

Psoriasis is an autoimmune skin disease characterized by hyperproliferation of keratinocytes due to interplay between keratinocytes and immune cells. Iron status plays an important role in modifying the function of the immune system. Heme oxygenase (HO), heme-degrading enzyme, plays important role in protective response to oxidative cellular stress. We aimed in this study to map the iron status and HO levels and declare the role HO enzyme in iron homeostasis and immune-modulation in psoriasis. Fifty-one patients with psoriasis and 50 age- and sex-matched healthy controls were enrolled in this study. 5 mL blood sample was withdrawn from each subject. Hepcidin, iron soluble transferring receptor (sTfR), and total iron binding capacity (TIBC) were estimated using ELISA technique and, HO-1 gene level was detected using RT-PCR (reverse transcription—polymerase chain reaction). Iron levels, TIBC, and hepcidin were significantly lower in cases compared to controls. On the contrary, sTfR and HO-1 were significantly over-expressed in cases compared to controls (p < 0.05 in all). HO-1 expression negatively correlated with PASI score and disease extent (%) (r = − 0.614-, p = 0.001; r = − 0.807-, p = 0.001 respectively). There were no significant associations between HO-1 expression and iron, TIBC, hepcidin, sTfR levels (p > 0.05 in all). Iron supplements for the patients with psoriasis are important to maintain haematopoiesis. The induction of HO-1 might have be a promising approach for the treatment of psoriasis through antioxidant ability, immunomodulatory role as well as its role in heme synthesis.

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Authors and Affiliations

  1. Dermatology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

    Abdel-Aziz A. El-Rifaie, Reham W. Doss & Mai A. Kamal

  2. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Dina Sabry

  3. Clinical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt

    Dalia M. Abd El Hassib

Authors
  1. Abdel-Aziz A. El-Rifaie
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  2. Dina Sabry
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  3. Reham W. Doss
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  4. Mai A. Kamal
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  5. Dalia M. Abd El Hassib
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Correspondence to Dina Sabry.

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The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the local research ethics committee of the Faculty of Medicine, Beni Suef University.

Informed consent

The purpose of the study was explained to each patient, and a written informed consent was taken before data collection.

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El-Rifaie, AA.A., Sabry, D., Doss, R.W. et al. Heme oxygenase and iron status in exosomes of psoriasis patients. Arch Dermatol Res 310, 651–656 (2018). https://doi.org/10.1007/s00403-018-1852-6

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  • DOI: https://doi.org/10.1007/s00403-018-1852-6

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