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Histological findings after argon plasma coagulation: an ex-vivo study revealing a possible role in superficial ablative treatment of the skin

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Abstract

Argon plasma coagulation (APC) is an electrosurgical technique which can be used to ablate skin lesions with limited invasion depth into dermal tissue. Hence, APC might be well suited for the removal of epithelial tumours. However, there are no data on the effects of APC on human skin tissue. Thus, the aim of this study was to determine the extent of epidermal and dermal damage after APC of human skin. We performed APC ex-vivo on 91 freshly resected human skin samples, which were obtained after reconstructive surgical closures in actinically damaged areas. Tissue effects were evaluated histologically and compared across different power settings. Using 15, 30, and 45 W, median (interquartile range; IQR) coagulation depths were 110.0 µm (91.7–130.0), 113.3 µm (85.8–135.0), and 130.0 µm (100.0–153.3.0), respectively. Median (IQR) thickness of necrosis zone was 30.0 µm (23.3–40.0) at 15 W, 26.7 µm (20.0–41.6) at 30 W, and 43.3 µm (30.8–57.5) at 45 W. The Kruskal–Wallis test showed significant differences between 15 and 30 W versus 45 W for coagulation depth (P = 0.0414), necrosis zone (P = 0.0017), and necrosis according to overlaying epidermal thickness (P = 0.0467). In summary, APC is a simple and controllable electrosurgical technique to remove epidermal tissue with limited penetration to the dermis. Thus, APC is particularly suited for the ablation of epithelial skin lesions and, therefore, may serve as possible treatment approach for intraepithelial neoplasms such as actinic keratosis.

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Authors and Affiliations

  1. Department of Dermatology, Venereology and Allergology, Dermatologic Surgery Unit, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany

    L. Schmitz, S. Hessam, L. Scholl, S. Reitenbach, M. H. Segert & F. G. Bechara

  2. Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany

    T. Gambichler & E. Stockfleth

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  1. L. Schmitz
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  2. S. Hessam
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  3. L. Scholl
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Corresponding author

Correspondence to F. G. Bechara.

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Conflict of interest

L. Schmitz: lecture fees from Almirall, Biofrontera, Galderma, Meda, and Riemser. S. Hessam: none. L. Scholl: none. S. Reitenbach: none. M.H. Segert: none. T. Gambichler: lecture fees from Novartis, Roche, NeraCare, and BMS. E. Stockfleth: lectures from Almirall, Leo, and Pierre Fabre. FG Bechara: none.

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Obtained (No.: #15-5524-BR).

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Schmitz, L., Hessam, S., Scholl, L. et al. Histological findings after argon plasma coagulation: an ex-vivo study revealing a possible role in superficial ablative treatment of the skin. Arch Dermatol Res 310, 157–163 (2018). https://doi.org/10.1007/s00403-018-1810-3

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  • DOI: https://doi.org/10.1007/s00403-018-1810-3

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