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Evaluating the accuracy of microRNA27b and microRNA137 as biomarkers of activity and potential malignant transformation in oral lichen planus patients

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Abstract

Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease with a potential malignant transformation, characterized by cytotoxic T cells against basal epithelial cells. MicroRNAs (MiRNAs) are short non-coding RNA that plays critical role in gene expression at post-transcriptional levels. Much evidence showed that miRNAs play an important role in regulating immune response and cancer development. The purpose of the present study was to compare the expression of miRNA 27b and miRNA 137 in tissues and saliva between OLP patients and controls by using RT-qPCR and to evaluate their use as biomarkers of disease activity and potential malignant transformation. Our results showed down expression of miRNA 27b and miRNA 137 in tissue and saliva of OLP patients compared to controls; among OLP subgroups, erosive-type miRNA 137 revealed the lowest level in tissue and saliva. In conclusion, alteration of miRNA 27b and miRNA 137 gene expression signify their use as biomarkers for diseases activity and tendency of malignant transformation, and down expression of miRNA 137 especially in erosive-type favors the use of saliva sample as a noninvasive method for monitoring a potential malignant transformation of OLP.

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Acknowledgements

I would like to express my deepest appreciation to my professors and colleagues in Faculty of Oral and Dental Medicine and in Faculty of Medicine, Cairo University-Egypt.

Funding

The study was funded by personal resources.

Author information

Authors and Affiliations

  1. Faculty of Dentistry, Aden University, Khormaksar, Aden, Yemen

    Sana Maher Hasan Aghbari

  2. Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

    Soheir Mohamed Gaafar & Shahira El Ashiry

  3. Faculty of Medicine, Cairo University, Cairo, Egypt

    Olfat Gamil Shaker

  4. Department of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

    Shaimaa Omar Zayed

Authors
  1. Sana Maher Hasan Aghbari
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  2. Soheir Mohamed Gaafar
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  3. Olfat Gamil Shaker
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  4. Shahira El Ashiry
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  5. Shaimaa Omar Zayed
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Corresponding author

Correspondence to Sana Maher Hasan Aghbari.

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The authors declare that they have no conflict of interest.

Ethical approval

This article was approved by the Faculty of Oral and Dental Medicine Research Ethics committee, Cairo University in September 2013.

Informed consent

Following an explanation of the study as well as information about the sampling procedures, each subject signed a written informed consent form approved by the Faculty Research Ethics committee.

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Aghbari, S.M.H., Gaafar, S.M., Shaker, O.G. et al. Evaluating the accuracy of microRNA27b and microRNA137 as biomarkers of activity and potential malignant transformation in oral lichen planus patients. Arch Dermatol Res 310, 209–220 (2018). https://doi.org/10.1007/s00403-018-1805-0

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  • DOI: https://doi.org/10.1007/s00403-018-1805-0

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