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Construction of a plasmid vector containing epidermal growth factor receptor and C-Jun shRNA

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Abstract

The objective of this study was to construct a plasmid vector for EGFR-hm-1 and C-Junh-825 small interfering RNA (siRNA). EGFR-hm-1 and C-Jun-hm-825 oligonucleotide fragments were synthesized and short hairpin RNA (shRNA) were amplified by PCR. Plasmids were isolated from E. coli TOP10 bacterium by restriction enzyme digestion using pst1 and BamH1 and oligonucleotide fragments were cloned into the pSilencer plasmid containing the U6 promoter. Recombinant clones were generated by transforming JM109 competent cells with plasmid vectors containing shRNA molecules. 58 base-paired EGFR-hm-1 and 59 base-paired C-Jun-hm-825 oligonucleotide fragments were isolated. The fragments were 100% homologous with human sequences available on GenBank. The recombinant pSilencer1.0 vector containing a 58-bp EGFR-hm-1 and 59-bp C-Jun-hm-825 fragment was constructed. These vectors have the potential to be used as treatment to combat skin photoaging under UV exposure.

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Authors and Affiliations

  1. Department of Plastic Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650103, Yunnan, China

    Hong Xiao, Fang Yang, Yanan Zhao & Yin Liu

  2. Department of Plastic Surgery, YESTAR Aesthetic Plastic Hospital, Kunming, Yunnan, China

    Ruinian Yang

Authors
  1. Hong Xiao
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  2. Ruinian Yang
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  3. Fang Yang
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  4. Yanan Zhao
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  5. Yin Liu
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Correspondence to Yin Liu.

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Xiao, H., Yang, R., Yang, F. et al. Construction of a plasmid vector containing epidermal growth factor receptor and C-Jun shRNA. Arch Dermatol Res 310, 241–243 (2018). https://doi.org/10.1007/s00403-017-1803-7

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  • DOI: https://doi.org/10.1007/s00403-017-1803-7

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