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Changes in circulating cell-free DNA and nucleosomes in patients with exacerbated psoriasis

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Abstract

Psoriasis is a multifactorial chronic inflammatory disease. We aimed to examine blood levels of nucleosomes derived from apoptotic cells, nucleosomal cell-free DNA (cfDNA) and immune-inflammatory biomarkers tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and interleukin 6 (IL-6) in psoriatic subjects. The study included 28 patients with exacerbated psoriasis vulgaris and 22 controls. The clinical and laboratory investigations included the determination of PASI score, BMI, cfDNA (by real-time PCR), nucleosomes, TNF-α, CRP, and IL-6. The range of PASI score in psoriatic patients was 10–34 (median 19). In the patients, we found significantly elevated levels (p < 0.001) of cfDNA, nucleosomes, TNF-α, CRP, and IL-6. We did not find any significant relationship between the analyzed parameters in either group (i.e., experimental or control). Elevated levels of the biomarkers of inflammation (TNF-α, CRP, and IL-6) and the indicators of apoptosis (cfDNA, circulating nucleosomes) proved that exacerbated psoriasis vulgaris is associated with a high degree of systemic inflammatory responses and dysregulated apoptotic pathways.

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Acknowledgements

The study was supported by the projects PROGRES Q40-09, Q40-10 and Q40-11 of Charles University, Faculty of Medicine in Hradec Kralove, Czech Republic.

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Authors and Affiliations

  1. Institute of Clinical Biochemistry and Diagnostics, Charles University Hospital and Faculty of Medicine in Hradec Kralove, 50005, Hradec Kralove, Czech Republic

    Martin Beranek, Marcela Chmelarova & Vladimir Palicka

  2. Department of Biochemical Sciences, Charles University, Faculty of Pharmacy in Hradec Kralove, 50005, Hradec Kralove, Czech Republic

    Martin Beranek

  3. Institute of Hygiene and Preventive Medicine, Charles University, Faculty of Medicine in Hradec Kralove, 50038, Hradec Kralove, Czech Republic

    Zdenek Fiala

  4. Institute of Pathological Physiology, Charles University, Faculty of Medicine in Hradec Kralove, Simkova 870, 50038, Hradec Kralove, Czech Republic

    Jan Kremlacek & Lenka Borska

  5. Institute of Clinical Immunology and Allergology, Charles University, Faculty of Medicine in Hradec Kralove, 50038, Hradec Kralove, Czech Republic

    Ctirad Andrys & Jan Krejsek

  6. Clinic of Dermal and Venereal Diseases, Charles University Hospital Hradec Kralove, 500 05, Hradec Kralove, Czech Republic

    Kvetoslava Hamakova

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  1. Martin Beranek
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Correspondence to Lenka Borska.

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All procedures performed in the study involving human participants were in accordance with the ethical standards of the Ethics Committee, University Hospital Hradec Kralove and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Beranek, M., Fiala, Z., Kremlacek, J. et al. Changes in circulating cell-free DNA and nucleosomes in patients with exacerbated psoriasis. Arch Dermatol Res 309, 815–821 (2017). https://doi.org/10.1007/s00403-017-1785-5

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  • DOI: https://doi.org/10.1007/s00403-017-1785-5

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