Abstract
Proteases and their inhibitors play an important role in epidermal homeostasis. Their imbalance contributes to severe skin diseases. SPINK7 is a member of the SPINK protease inhibitor family and has been described so far as a cancer-related gene in the esophagus. Herein, we describe for the first time its expression in healthy human skin. Moreover, SPINK7 is up-regulated in inflammatory skin diseases like psoriasis and eczema as demonstrated by immunohistochemistry, though real-time PCR analyses revealed no significant up-regulation. In cultured keratinocytes, SPINK7 mRNA expression was up-regulated by IL-17A together with IFNγ. Our observation points to a role of SPINK7 in skin homeostasis and its involvement in inflammatory skin diseases.
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Abbreviations
- GAPDH:
-
Glyceraldehyde dehydrogenase
- KLK:
-
Kallikrein-related peptidase
- NHEC:
-
Natural human epidermal keratinocytes
- SPINK:
-
Serine protease inhibitor of Kazal-type
- LEKTI:
-
Lympho-epithelial Kazal-type inhibitor
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Acknowledgements
This work was part of the medical doctor thesis of Clemens Weber.
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Deutsche Forschungsgemeinschaft Me2037/3-3 (UMH) and the National Natural Science Foundation of China No.81472866 (ZW).
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This article does not contain any studies with human participants or animals performed by any of the authors.
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Biopsies of patients were taken due to obtain dermatological diagnoses at the Department of Dermatology, University Kiel. Informed consent was obtained from all individual participants included in the study.
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Weber, C., Fischer, J., Redelfs, L. et al. The serine protease inhibitor of Kazal-type 7 (SPINK7) is expressed in human skin. Arch Dermatol Res 309, 767–771 (2017). https://doi.org/10.1007/s00403-017-1773-9
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DOI: https://doi.org/10.1007/s00403-017-1773-9