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The serine protease inhibitor of Kazal-type 7 (SPINK7) is expressed in human skin

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Abstract

Proteases and their inhibitors play an important role in epidermal homeostasis. Their imbalance contributes to severe skin diseases. SPINK7 is a member of the SPINK protease inhibitor family and has been described so far as a cancer-related gene in the esophagus. Herein, we describe for the first time its expression in healthy human skin. Moreover, SPINK7 is up-regulated in inflammatory skin diseases like psoriasis and eczema as demonstrated by immunohistochemistry, though real-time PCR analyses revealed no significant up-regulation. In cultured keratinocytes, SPINK7 mRNA expression was up-regulated by IL-17A together with IFNγ. Our observation points to a role of SPINK7 in skin homeostasis and its involvement in inflammatory skin diseases.

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Abbreviations

GAPDH:

Glyceraldehyde dehydrogenase

KLK:

Kallikrein-related peptidase

NHEC:

Natural human epidermal keratinocytes

SPINK:

Serine protease inhibitor of Kazal-type

LEKTI:

Lympho-epithelial Kazal-type inhibitor

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Acknowledgements

This work was part of the medical doctor thesis of Clemens Weber.

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Authors and Affiliations

  1. Department of Dermatology, Universitätsklinikum Schleswig-Holstein, Campus Kiel, Schittenhelmstr. 7, 24105, Kiel, Germany

    Clemens Weber, Jan Fischer, Lisa Redelfs, Franziska Rademacher, Jürgen Harder, Stephan Weidinger, Zhihong Wu & Ulf Meyer-Hoffert

  2. Institute of Biochemistry and Cell Biology, Zhejiang University of Science and Technology, Hangzhou, 310023, China

    Zhihong Wu

Authors
  1. Clemens Weber
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  2. Jan Fischer
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  3. Lisa Redelfs
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  4. Franziska Rademacher
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  5. Jürgen Harder
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  6. Stephan Weidinger
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  7. Zhihong Wu
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  8. Ulf Meyer-Hoffert
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Corresponding author

Correspondence to Ulf Meyer-Hoffert.

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The authors declare that they have no conflict of interest.

Funding

Deutsche Forschungsgemeinschaft Me2037/3-3 (UMH) and the National Natural Science Foundation of China No.81472866 (ZW).

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Biopsies of patients were taken due to obtain dermatological diagnoses at the Department of Dermatology, University Kiel. Informed consent was obtained from all individual participants included in the study.

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Weber, C., Fischer, J., Redelfs, L. et al. The serine protease inhibitor of Kazal-type 7 (SPINK7) is expressed in human skin. Arch Dermatol Res 309, 767–771 (2017). https://doi.org/10.1007/s00403-017-1773-9

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  • DOI: https://doi.org/10.1007/s00403-017-1773-9

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